Carvalho Cristina, Moreira Paula I
CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
Institute for Interdisciplinary Research, University of Coimbra, Coimbra, Portugal.
Bio Protoc. 2017 Sep 5;7(17):e2535. doi: 10.21769/BioProtoc.2535.
The prevalence of neurodegenerative diseases is increasing worldwide. Cerebrovascular disorders and/or conditions known to affect brain vasculature, such as diabetes, are well-known risk factors for neurodegenerative diseases. Thus, the evaluation of the brain vasculature is of great importance to better understand the mechanisms underlying brain damage. We established a protocol for the isolation of brain vessels from rodents. This is a simple, non-enzymatic isolation protocol that allows us to perform comparative studies in different animal models of disease, helping understand the impact of several pathological conditions on brain vasculature and how those alterations predispose to neurodegenerative conditions.
神经退行性疾病在全球范围内的患病率正在上升。脑血管疾病和/或已知会影响脑血管系统的病症，如糖尿病，是神经退行性疾病的众所周知的危险因素。因此，评估脑血管系统对于更好地理解脑损伤的潜在机制非常重要。我们建立了一种从啮齿动物中分离脑血管的方案。这是一种简单的非酶分离方案，使我们能够在不同的疾病动物模型中进行比较研究，有助于了解几种病理状况对脑血管系统的影响以及这些改变如何导致神经退行性疾病。
