Bartha Gergely Sámuel, Tóth Gergő, Horváth Péter, Kiss Eszter, Papp Nóra, Kerényi Monika
Department of Pharmacognosy, University of Pécs, Rókus u. 2, Pécs, H-7624, Hungary.
Department of Pharmaceutical Chemistry, Semmelweis University, Hőgyes Endre u. 9, Budapest, H-1092, Hungary.
Biol Futur. 2019 Dec;70(4):323-329. doi: 10.1556/019.70.2019.36. Epub 2019 Dec 1.
Several Aristolochia species were used as medicinal herb across Europe and in recent years, their antimicrobial activity has also been investigated.
In this study, A. clematitis was selected to evaluate the aristolochic acids I and II (AA I and AA II) concentrations and the antimicrobial activity of methanol, hexane, butanol, and ethyl acetate extracts of the root, stem, leaf, root, and fruit. AA I and AA II contents were measured by a validated high-performance liquid chromatography-ultraviolet method.
Each fraction of the plant contained AA I and AA II and the root was found to have the highest contents of AA I (1.09%) and AA II (0.7454%). The minimum inhibitory concentrations of all extracts were determined by standard microdilution method. The fruit's extracts showed the most efficient antimicrobial effect against both methicillin sensitive and resistant Staphylococcus aureus strains.
Correlation between the AA I and AA II concentrations and the antimicrobial effect was not found.
欧洲曾将多种马兜铃属植物用作草药，近年来，人们也对其抗菌活性展开了研究。
本研究选取了铁线莲状马兜铃，以评估其根、茎、叶、根和果实的甲醇、己烷、丁醇和乙酸乙酯提取物中马兜铃酸I和II（AA I和AA II）的浓度及抗菌活性。采用经过验证的高效液相色谱-紫外法测定AA I和AA II的含量。
该植物的各个部位均含有AA I和AA II，其中根中AA I（1.09%）和AA II（0.7454%）的含量最高。通过标准微量稀释法测定所有提取物的最低抑菌浓度。果实提取物对甲氧西林敏感和耐药金黄色葡萄球菌菌株均显示出最有效的抗菌效果。
未发现AA I和AA II浓度与抗菌效果之间存在相关性。
