Center of Clinical Laboratory Medicine, The Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Zhejiang, China.
J Clin Lab Anal. 2021 Nov;35(11):e24016. doi: 10.1002/jcla.24016. Epub 2021 Sep 23.
Recently, the rise of syphilitic seroresistance brings great confusion to the clinical diagnosis and treatment of syphilis, and no clear diagnostic marker has been found to distinguish syphilitic seroresistance from other progression of syphilis. This study evaluated the serum chemokines levels of CCL2, CXCL8, CXCL9, and CXCL10 and its correlation with blood routine, coagulation, and biochemical indexes in seroresistant syphilitic patients.
Serum levels of chemokines were quantitatively determined by Flow Cytometric Bead Array (CBA). The results expressed in pg/ml. Clinical parameters were detected and analyzed according to the clinical laboratory standards. A correlation analysis was subsequently performed.
The seroresistant syphilitic patients increased significantly serum chemokines levels of CXCL8 (***p < 0.001), CXCL9 (***p < 0.001), and CXCL10 (**p < 0.01) when compared to noninfected individuals, but the CCL2 was not statistically significant, and serum CXCL8 shows a strong association with platelets (r = 0.51, **p = 0.004) and serum CXCL10 was significantly positively related to INR levels (r = 0.49, **p = 0.007).
Increasing serum abnormalities in CXCL8, CXCL9, and CXCL10 level combining with platelets of peripheral blood and plasmatic INR in syphilis patients may be helpful for the diagnosis of serofast state.
最近，梅毒血清学耐药的出现给梅毒的临床诊断和治疗带来了很大的困惑，尚未发现明确的诊断标志物来区分梅毒血清学耐药与梅毒的其他进展。本研究评估了血清趋化因子 CCL2、CXCL8、CXCL9 和 CXCL10 的水平及其与血清学耐药梅毒患者血常规、凝血和生化指标的相关性。
采用流式细胞术微珠阵列（CBA）定量测定趋化因子水平。结果以 pg/ml 表示。根据临床实验室标准检测和分析临床参数。随后进行相关性分析。
与未感染者相比，血清学耐药的梅毒患者的血清趋化因子 CXCL8（***p<0.001）、CXCL9（***p<0.001）和 CXCL10（**p<0.01）水平显著升高，但 CCL2 无统计学意义，血清 CXCL8 与血小板呈强相关性（r=0.51，**p=0.004），血清 CXCL10 与 INR 水平呈显著正相关（r=0.49，**p=0.007）。
梅毒患者外周血血小板和血浆 INR 中 CXCL8、CXCL9 和 CXCL10 水平的异常增加可能有助于诊断血清固定状态。
