Ouyang Dan, Wang Lan-Chun, Tang Ting, Feng Hong
Key Laboratory of Molecular Biology and Biotechnology of Sichuan Province, Department of Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, China.
Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Department of Biotechnology, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China.
Plants (Basel). 2021 Sep 17;10(9):1934. doi: 10.3390/plants10091934.
Oliver is a woody plant with great economic and medicinal value. Its dried bark has a long history of use as a traditional medicinal material in East Asia, which led to many glycosides, such as aucubin, geniposide, hyperoside, astragalin, and pinoresinol diglucoside, being recognized as pharmacologically active ingredients. Uridine diphosphate glycosyltransferases (UGTs) catalyze a glycosyl-transferring reaction from the donor molecule uridine-5'-diphosphate-glucose (UDPG) to the substrate, which plays an important role in many biological processes, such as plant growth and development, secondary metabolism, and environmental adaptation. In order to explore the biosynthetic pathways of glycosides in , 91 putative UGT genes were identified throughout the complete genome of through function annotation and an UDPGT domain search. Phylogenetic analysis categorized them into 14 groups. We also performed GO annotations on all the UGTs to gain insights into their functions in . In addition, transcriptomic analysis indicated that most UGTs showed different expression patterns across diverse organs and various growing seasons. By protein-protein interaction predication, a biosynthetic routine of flavonoids and their glycosides was also proposed. Undoubtedly, these results will help in future research into the biosynthetic pathways of glycoside compounds in .
齐墩果是一种具有很高经济和药用价值的木本植物。其干燥树皮在东亚作为传统药材已有悠久的使用历史，这使得许多糖苷，如桃叶珊瑚苷、栀子苷、金丝桃苷、紫云英苷和松脂醇二葡萄糖苷，被认为是具有药理活性的成分。尿苷二磷酸糖基转移酶（UGTs）催化从供体分子尿苷-5'-二磷酸葡萄糖（UDPG）到底物的糖基转移反应，该反应在许多生物过程中发挥重要作用，如植物生长发育、次生代谢和环境适应。为了探索齐墩果中糖苷的生物合成途径，通过功能注释和UDPGT结构域搜索，在齐墩果的全基因组中鉴定出91个推定的UGT基因。系统发育分析将它们分为14组。我们还对所有UGT进行了基因本体论（GO）注释，以深入了解它们在齐墩果中的功能。此外，转录组分析表明，大多数UGT在不同器官和不同生长季节表现出不同的表达模式。通过蛋白质-蛋白质相互作用预测，还提出了黄酮类化合物及其糖苷的生物合成途径。毫无疑问，这些结果将有助于未来对齐墩果中糖苷化合物生物合成途径的研究。
