Department of Intensive Care Medicine, Inselspital, Bern University Hospital, University of Bern, 3010, Bern, Switzerland.
Institute for Infectious Diseases, University of Bern, Bern, Switzerland.
BMC Res Notes. 2021 Sep 27;14(1):381. doi: 10.1186/s13104-021-05796-1.
Bacteriophages (or phages) are viruses which infect and lyse bacteria. The therapeutic use of phages (phage therapy) has regained attention in the last decades as an alternative strategy to treat infections caused by antimicrobial-resistant bacteria. In clinical settings it is most likely that phages are administered adjunct to antibiotics. For successful phage therapy it is therefore crucial to investigate different phage-antibiotic combinations in vivo. This study aimed to elucidate the combinatorial effects of systemic daptomycin and nebulised bacteriophages for the treatment of experimental pneumonia due to methicillin-resistant Staphylococcus aureus (MRSA).
Using a rat model of ventilator-associated pneumonia caused by MRSA, the simultaneous application of intravenous daptomycin and nebulised phages was not superior to aerophage therapy alone at improving animal survival (55% vs. 50%), or reducing bacterial burdens in the lungs, or spleen. Thus, this combination does not seem to be of benefit for use in patients with MRSA pneumonia.
噬菌体（或 phages）是感染并裂解细菌的病毒。在过去几十年中，噬菌体（噬菌体疗法）作为一种治疗抗微生物药物耐药细菌引起的感染的替代策略重新受到关注。在临床环境中，噬菌体最有可能与抗生素联合使用。因此，成功进行噬菌体治疗的关键是在体内研究不同的噬菌体-抗生素组合。本研究旨在阐明系统万古霉素和雾化噬菌体联合治疗耐甲氧西林金黄色葡萄球菌（MRSA）引起的实验性肺炎的组合效应。
使用耐甲氧西林金黄色葡萄球菌引起的呼吸机相关性肺炎大鼠模型，静脉万古霉素和雾化噬菌体的同时应用在提高动物存活率（55%对 50%）、降低肺部或脾脏细菌负荷方面并不优于单纯 aerophage 治疗。因此，这种组合似乎对治疗耐甲氧西林金黄色葡萄球菌肺炎的患者没有益处。
