克隆用于大环内酯类抗生素生物合成的基因。
Cloning genes for the biosynthesis of a macrolide antibiotic.
作者信息
Fishman S E, Cox K, Larson J L, Reynolds P A, Seno E T, Yeh W K, Van Frank R, Hershberger C L
机构信息
Lilly Corporate Center, Indianapolis, IN 46285.
出版信息
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8248-52. doi: 10.1073/pnas.84.23.8248.
Abstract
Macrocin-O-methyltransferase (MacOMeTase) catalyzes the final enzymatic step in the biosynthesis of tylosin in Streptomyces fradiae. A 44-base mixed oligonucleotide probe containing only guanosine and cytidine in the third position of degenerate codons was synthesized based on the amino acid sequence of the amino terminus of MacOMeTase. Plaque blot hybridization to a bacteriophage lambda library and colony blot hybridization to a cosmid library of S. fradiae DNA identified recombinants that contained overlapping fragments of chromosomal DNA. The nucleotide sequence of the cloned DNA verified that the DNA contained the coding sequence for MacOMeTase. Recombinant plasmids transformed mutants blocked in tylosin biosynthesis and complemented tylF (the structural gene for MacOMeTase) and tyl mutations of eight other classes.
摘要
大环菌素 - O - 甲基转移酶（MacOMeTase）催化弗氏链霉菌中泰乐菌素生物合成的最后一步酶促反应。基于MacOMeTase氨基末端的氨基酸序列，合成了一个44个碱基的混合寡核苷酸探针，该探针在简并密码子的第三位仅含有鸟苷和胞苷。与弗氏链霉菌DNA的λ噬菌体文库进行噬菌斑杂交以及与粘粒文库进行菌落杂交，鉴定出含有染色体DNA重叠片段的重组体。克隆DNA的核苷酸序列证实该DNA包含MacOMeTase的编码序列。重组质粒转化在泰乐菌素生物合成中受阻的突变体，并互补了tylF（MacOMeTase的结构基因）和其他八类tyl突变。
相似文献
1
Cloning genes for the biosynthesis of a macrolide antibiotic.克隆用于大环内酯类抗生素生物合成的基因。
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8248-52. doi: 10.1073/pnas.84.23.8248.
2
The use of recombinant DNA techniques to study tylosin biosynthesis and resistance in Streptomyces fradiae.利用重组DNA技术研究弗氏链霉菌中泰乐菌素的生物合成及抗性。
J Nat Prod. 1986 Nov-Dec;49(6):971-80. doi: 10.1021/np50048a002.
3
Properties of Streptomyces fradiae mutants blocked in biosynthesis of the macrolide antibiotic tylosin.在大环内酯类抗生素泰乐菌素生物合成中受阻的弗氏链霉菌突变体的特性。
Antimicrob Agents Chemother. 1981 Aug;20(2):214-25. doi: 10.1128/AAC.20.2.214.
4
Properties of S-adenosyl-L-methionine:macrocin O-methyltransferase in extracts of Streptomyces fradiae strains which produce normal or elevated levels of tylosin and in mutants blocked in specific O-methylations.弗氏链霉菌菌株提取物中S-腺苷-L-甲硫氨酸：大环菌素O-甲基转移酶的特性，这些菌株产生正常或高水平的泰乐菌素，以及在特定O-甲基化中受阻的突变体。
Antimicrob Agents Chemother. 1981 Sep;20(3):370-7. doi: 10.1128/AAC.20.3.370.
5
S-Adenosyl-L-methionine: macrocin O-methyltransferase activities in a series of Streptomyces fradiae mutants that produce different levels of the macrolide antibiotic tylosin.S-腺苷-L-甲硫氨酸：弗氏链霉菌一系列产生不同水平大环内酯类抗生素泰乐菌素的突变体中的大菌素O-甲基转移酶活性。
Antimicrob Agents Chemother. 1982 May;21(5):758-63. doi: 10.1128/AAC.21.5.758.
6
Cloning and expression of a tylosin resistance gene from a tylosin-producing strain of Streptomyces fradiae.来自弗氏链霉菌泰乐菌素生产菌株的泰乐菌素抗性基因的克隆与表达。
Mol Gen Genet. 1986 Sep;204(3):532-9. doi: 10.1007/BF00331036.
7
8
The tylosin biosynthetic cluster from Streptomyces fradiae: genetic organization of the left region.弗氏链霉菌泰乐菌素生物合成基因簇：左侧区域的基因组织
Microbiology (Reading). 1999 Apr;145 ( Pt 4):855-868. doi: 10.1099/13500872-145-4-855.
9
High frequency conjugal transfer of tylosin genes and amplifiable DNA in Streptomyces fradiae.弗氏链霉菌中泰乐菌素基因和可扩增DNA的高频接合转移
Mol Gen Genet. 1986 Mar;202(3):348-55. doi: 10.1007/BF00333261.
10
Cloning of the macrolide antibiotic biosynthesis gene acyA, which encodes 3-O-acyltransferase, from Streptomyces thermotolerans and its use for direct fermentative production of a hybrid macrolide antibiotic.从耐热链霉菌中克隆编码3 - O - 酰基转移酶的大环内酯类抗生素生物合成基因acyA及其在直接发酵生产杂合大环内酯类抗生素中的应用。
Appl Environ Microbiol. 1994 Jul;60(7):2657-60. doi: 10.1128/aem.60.7.2657-2660.1994.
引用本文的文献
1
Chemo-enzymatic total synthesis: current approaches toward the integration of chemical and enzymatic transformations.化学酶法全合成：化学与酶促转化整合的当前方法
Beilstein J Org Chem. 2024 Jul 23;20:1693-1712. doi: 10.3762/bjoc.20.151. eCollection 2024.
2
A High-Throughput Method Based on Microculture Technology for Screening of High-Yield Strains of Tylosin-Producing .基于微培养技术的高效筛选泰乐菌素高产菌株的方法
J Microbiol Biotechnol. 2023 Jun 28;33(6):831-839. doi: 10.4014/jmb.2210.10023. Epub 2023 Mar 10.
3
Functional insights into the mode of DNA and ligand binding of the TetR family regulator TylP from .来自……的TetR家族调控因子TylP的DNA和配体结合模式的功能见解 。
你提供的原文似乎不完整，句末“from.”后面缺少具体信息。
J Biol Chem. 2017 Sep 15;292(37):15301-15311. doi: 10.1074/jbc.M117.788000. Epub 2017 Jul 24.
4
Biosynthesis of antibiotics in streptomycetes.链霉菌中抗生素的生物合成。
World J Microbiol Biotechnol. 1992 Dec;8 Suppl 1:83-6. doi: 10.1007/BF02421502.
5
Deletion analysis of the avermectin biosynthetic genes of Streptomyces avermitilis by gene cluster displacement.通过基因簇置换对阿维链霉菌阿维菌素生物合成基因进行缺失分析。
J Bacteriol. 1993 May;175(9):2552-63. doi: 10.1128/jb.175.9.2552-2563.1993.
6
The impact of genetic engineering on the commercial production of antibiotics by Streptomyces and related bacteria.基因工程对链霉菌及相关细菌抗生素商业生产的影响。
Appl Biochem Biotechnol. 1987 Sep-Dec;16:169-90. doi: 10.1007/BF02798365.
7
Cloning and expression of the Mycobacterium bovis BCG gene for extracellular alpha antigen.牛分枝杆菌卡介苗细胞外α抗原基因的克隆与表达
J Bacteriol. 1988 Sep;170(9):3847-54. doi: 10.1128/jb.170.9.3847-3854.1988.
8
Gene cluster containing the genes for tyrocidine synthetases 1 and 2 from Bacillus brevis: evidence for an operon.包含来自短短芽孢杆菌的短杆菌酪肽合成酶1和2基因的基因簇：操纵子的证据。
J Bacteriol. 1989 Sep;171(9):4881-7. doi: 10.1128/jb.171.9.4881-4887.1989.
9
Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythreus).糖多孢红霉菌（红霉素链霉菌）中红霉素生物合成途径脱氧糖部分相关基因的克隆
J Bacteriol. 1989 Nov;171(11):5872-81. doi: 10.1128/jb.171.11.5872-5881.1989.
10
Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase.编码去乙酰氧头孢菌素C合成酶的棒状链霉菌基因在大肠杆菌中的克隆、特性鉴定及表达
J Bacteriol. 1989 Feb;171(2):754-60. doi: 10.1128/jb.171.2.754-760.1989.
本文引用的文献
1
Transduction of linked genetic characters of the host by bacteriophage P1.噬菌体P1对宿主连锁遗传性状的转导
Virology. 1955 Jul;1(2):190-206. doi: 10.1016/0042-6822(55)90016-7.
2
Scientific evidence on the role of Ayurvedic herbals on bioavailability of drugs.关于阿育吠陀草药对药物生物利用度作用的科学证据。
J Ethnopharmacol. 1981 Sep;4(2):229-32. doi: 10.1016/0378-8741(81)90037-4.
3
Properties of S-adenosyl-L-methionine:macrocin O-methyltransferase in extracts of Streptomyces fradiae strains which produce normal or elevated levels of tylosin and in mutants blocked in specific O-methylations.弗氏链霉菌菌株提取物中S-腺苷-L-甲硫氨酸：大环菌素O-甲基转移酶的特性，这些菌株产生正常或高水平的泰乐菌素，以及在特定O-甲基化中受阻的突变体。
Antimicrob Agents Chemother. 1981 Sep;20(3):370-7. doi: 10.1128/AAC.20.3.370.
4
Properties of Streptomyces fradiae mutants blocked in biosynthesis of the macrolide antibiotic tylosin.在大环内酯类抗生素泰乐菌素生物合成中受阻的弗氏链霉菌突变体的特性。
Antimicrob Agents Chemother. 1981 Aug;20(2):214-25. doi: 10.1128/AAC.20.2.214.
5
A gas-liquid solid phase peptide and protein sequenator.一种气-液-固相肽和蛋白质测序仪。
J Biol Chem. 1981 Aug 10;256(15):7990-7.
6
Biosynthesis of the macrolide antibiotic tylosin. A preferred pathway from tylactone to tylosin.大环内酯类抗生素泰乐菌素的生物合成。从泰乐内酯到泰乐菌素的优选途径。
J Antibiot (Tokyo). 1983 Feb;36(2):131-41. doi: 10.7164/antibiotics.36.131.
7
High-performance liquid chromatographic assay for S-adenosyl-L-methionine: macrocin O-methyltransferase.S-腺苷-L-甲硫氨酸：大环菌素O-甲基转移酶的高效液相色谱测定法
J Chromatogr. 1984 Apr 20;288(1):157-65. doi: 10.1016/s0021-9673(01)93690-8.
8
Cloning of a Streptomyces gene for an O-methyltransferase involved in antibiotic biosynthesis.克隆参与抗生素生物合成的O-甲基转移酶的链霉菌基因。
Mol Gen Genet. 1983;190(3):394-8. doi: 10.1007/BF00331065.
9
Unstable genetic determinant of A-factor biosynthesis in streptomycin-producing organisms: cloning and characterization.链霉素产生菌中A因子生物合成的不稳定遗传决定因素：克隆与特性分析
J Bacteriol. 1984 May;158(2):481-7. doi: 10.1128/jb.158.2.481-487.1984.
10
Integrated DNA sequences in three streptomycetes form related autonomous plasmids after transfer to Streptomyces lividans.三种链霉菌中的整合DNA序列在转移至变铅青链霉菌后形成相关的自主质粒。
Plasmid. 1984 Jan;11(1):1-16. doi: 10.1016/0147-619x(84)90002-7.
Zotero 插件