Conn G, Hatcher V B
Biochem Biophys Res Commun. 1984 Oct 15;124(1):262-8. doi: 10.1016/0006-291x(84)90946-x.
Two anionic polypeptides which stimulate the proliferation of human umbilical vein endothelial cells have been isolated and purified to homogeneity from human brain using heparin affinity chromatography. The molecular weights of the polypeptides are 18,500 and 19,300; the isoelectric point for both polypeptides is at pH 5.2. The purified polypeptides differed in their ability to stimulate human endothelial cell growth. The half maximal activities observed for the 18.5 and 19.3 kilodalton polypeptides were at concentrations of 10.0 ng/ml and 0.9 ng/ml respectively.
利用肝素亲和层析法，从人脑中分离并纯化出两种能刺激人脐静脉内皮细胞增殖的阴离子多肽，使其达到同质状态。这两种多肽的分子量分别为18,500和19,300；它们的等电点均为pH 5.2。纯化后的多肽在刺激人内皮细胞生长的能力上有所不同。观察到18.5千道尔顿和19.3千道尔顿多肽的半最大活性分别在浓度为10.0 ng/ml和0.9 ng/ml时出现。
