莱茵衣藻运动的准弹性光散射和电影摄影研究。
A quasi-elastic light scattering and cinematographic investigation of motile Chlamydomonas reinhardtii.
作者信息
Racey T J, Hallett R, Nickel B
出版信息
Biophys J. 1981 Sep;35(3):557-71. doi: 10.1016/S0006-3495(81)84812-6.
Abstract
Quasi-elastic light scattering and cinematographical techniques were used to investigate the motility of Chlamydomonas reinhardtii (wild type). It was found that quantitative information on the trajectory of motion was required for a meaningful interpretation of the autocorrelation functions. Two models for describing the oscillatory motion of the cell were developed; one based on the instantaneous forward-and-backward motion of the cell, and the other based on a sinusoidal perturbation to the average forward motion. Both models gave satisfactory agreement with the shape of the experimentally measured autocorrelation function, thus making it possible to use this measurement to determine mean progressive swimming velocities in a population of greater than 200 cells.
摘要
利用准弹性光散射和电影摄影技术研究莱茵衣藻（野生型）的运动性。结果发现，为了对自相关函数进行有意义的解释，需要有关运动轨迹的定量信息。开发了两种描述细胞振荡运动的模型；一种基于细胞的瞬时前后运动，另一种基于对平均向前运动的正弦扰动。两种模型都与实验测量的自相关函数的形状给出了令人满意的一致性，从而使得利用这种测量来确定超过200个细胞群体中的平均前进游动速度成为可能。
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