Lambrou M, Nowotny H, Lutz D, Schweizer D
Cancer Genet Cytogenet. 1986 Oct;23(2):115-25. doi: 10.1016/0165-4608(86)90411-5.
Chromosome studies of a case of erythroleukemia in a 57-year-old female patient were made from bone marrow aspirates using the fluorescent primary stain/counterstain methodology. The chromosome number ranged from 42 to 110. There was a high proportion of hypotetraploid cells and a few hypertetraploid and hypooctaploid ones. Structurally normal chromosomes varied in number from cell to cell, ranging from one to seven in the polyploid cells. A number of marker chromosomes were observed, some of which occurred repeatedly in two copies per hypotetraploid cell. The chromosomes involved in aberrations were tentatively identified as #3, #5, #7, #12, #13, #15, #16, #18, #19, and #21. In the abnormal chromosome #16, which was missing a normal short arm, a new kind of heterochromatin was demonstrated by sequential staining with DA-DAPI and DAPI-AMD, suggesting de novo amplification of an A-T-rich satellite DNA sequence.
采用荧光初染/复染方法，对一名57岁女性红白血病患者的骨髓穿刺样本进行了染色体研究。染色体数目在42到110之间。亚四倍体细胞比例较高，还有一些超四倍体和亚八倍体细胞。结构正常的染色体数量在不同细胞间有所变化，多倍体细胞中从1条到7条不等。观察到一些标记染色体，其中一些在每个亚四倍体细胞中以两条重复出现。初步确定涉及畸变的染色体为#3、#5、#7、#12、#13、#15、#16、#18、#19和#21。在缺失正常短臂的异常染色体#16中，通过DA-DAPI和DAPI-AMD顺序染色显示出一种新型异染色质，提示富含A-T的卫星DNA序列发生了从头扩增。
