Department of Nuclear Medicine, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, 250014, China.
Biomedical Sciences College & Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, 250062, China.
Commun Biol. 2021 Oct 21;4(1):1207. doi: 10.1038/s42003-021-02742-6.
Numbers of nuclear speckles and paraspeckles components have been demonstrated to regulate herpes simplex virus 1 (HSV-1) replication. However, how HSV-1 infection affects the two nuclear bodies, and whether this influence facilitates the expression of viral genes, remains elusive. In the current study, we found that HSV-1 infection leads to a redistribution of speckles and paraspeckles components. Serine/arginine-rich splicing factor 2 (SRSF2), the core component of speckles, was associated with multiple paraspeckles components, including nuclear paraspeckles assembly transcript 1 (NEAT1), PSPC1, and P54nrb, in HSV-1 infected cells. This association coordinates the transcription of viral genes by binding to the promoters of these genes. By association with the enhancer of zeste homolog 2 (EZH2) and P300/CBP complex, NEAT1 and SRSF2 influenced the histone modifications located near viral genes. This study elucidates the interplay between speckles and paraspeckles following HSV-1 infection and provides insight into the mechanisms by which HSV-1 utilizes host cellular nuclear bodies to facilitate its life cycle.
核斑点和核旁斑点组件的数量已被证明可调节单纯疱疹病毒 1（HSV-1）的复制。然而，HSV-1 感染如何影响这两个核体，以及这种影响是否有助于病毒基因的表达，仍然难以捉摸。在本研究中，我们发现 HSV-1 感染导致斑点和核旁斑点组件重新分布。斑点的核心成分丝氨酸/精氨酸丰富剪接因子 2（SRSF2）与多个核旁斑点成分相关联，包括核旁斑点组装转录物 1（NEAT1）、PSPC1 和 P54nrb，在 HSV-1 感染的细胞中。这种关联通过与这些基因的启动子结合来协调病毒基因的转录。通过与增强子结合锌指蛋白 2（EZH2）和 P300/CBP 复合物的关联，NEAT1 和 SRSF2 影响位于病毒基因附近的组蛋白修饰。本研究阐明了 HSV-1 感染后斑点和核旁斑点之间的相互作用，并深入了解了 HSV-1 利用宿主细胞核体促进其生命周期的机制。
