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来源于香港牡蛎的新型抗氧化肽可改善 UV 诱导 HaCaT 细胞的光氧化。

Novel Antioxidant Peptides from Crassostrea Hongkongensis Improve Photo-Oxidation in UV-Induced HaCaT Cells.

机构信息

College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.

Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China.

出版信息

Mar Drugs. 2022 Jan 24;20(2):100. doi: 10.3390/md20020100.

DOI:10.3390/md20020100
PMID:35200629
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8875629/
Abstract

Enzymatic hydrolysates from Oysters (OAH) display multiple biological activities. Previously, a 3~5 KDa oyster ultrafiltration component (OUP) showed a high property of preventing skin oxidation. Subsequently, we identified specific peptides with such activity. OUP was fractionated stepwise by Sephadex-G25 and RP-HPLC, and active fractions were screened using UV-irradiated HaCaT cells. The most active fractions (OP5-3) were analyzed by LC-MS/MS and a total of 17 peptides were identified. Results from mass spectrometry showed that OP5-3 consisted of peptides with a molecular weight range of 841.51-1786.92 Da. Six of these peptides were synthesized for validating the activity of resisting skin oxidation in the same cell model. All six peptides showed varying degrees of antioxidant activity, while pretreatment of HaCaT cells with AIVAEVNEAAK alleviated UV cytotoxicity, inhibited metalloproteinase 1 (MMP-1) expression, and showed the highest activity to resist UV-induced skin photo-oxidation among these peptides. In addition, results from molecular docking analysis of MMP-1 with AIVAEVNEAAK showed that AIVAEVNEAAK suppresses its enzymatic activity by directly interacting with MMP-1 and thus exhibit anti-photoaging activity.

摘要

牡蛎酶解产物（OAH）具有多种生物学活性。先前，一种 3~5 kDa 的牡蛎超滤成分（OUP）表现出很高的皮肤抗氧化性能。随后，我们鉴定出具有这种活性的特定肽。OUP 采用 Sephadex-G25 和 RP-HPLC 分步分级，并用紫外线照射的 HaCaT 细胞筛选活性级分。最活跃的级分（OP5-3）通过 LC-MS/MS 进行分析，共鉴定出 17 种肽。质谱结果表明，OP5-3 由分子量在 841.51-1786.92 Da 的肽组成。其中 6 种肽被合成用于验证在相同细胞模型中抵抗皮肤氧化的活性。这 6 种肽都表现出不同程度的抗氧化活性，而 AIVAEVNEAAK 预处理 HaCaT 细胞可减轻 UV 细胞毒性、抑制金属蛋白酶 1（MMP-1）表达，并在这些肽中表现出最高的抵抗 UV 诱导皮肤光氧化的活性。此外，MMP-1 与 AIVAEVNEAAK 的分子对接分析结果表明，AIVAEVNEAAK 通过直接与 MMP-1 相互作用抑制其酶活性，从而表现出抗光老化活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d2e/8875629/0a344fc5e126/marinedrugs-20-00100-g015a.jpg
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