Lianou Daphne T, Petinaki Efthymia, Cripps Peter J, Gougoulis Dimitris A, Michael Charalambia K, Tsilipounidaki Katerina, Skoulakis Anargyros, Katsafadou Angeliki I, Vasileiou Natalia G C, Giannoulis Themis, Voidarou Chrysoula, Mavrogianni Vasia S, Caroprese Mariangela, Fthenakis George C
Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece.
University Hospital of Larissa, 41110 Larissa, Greece.
Biology (Basel). 2021 Oct 9;10(10):1016. doi: 10.3390/biology10101016.
The objectives of this work were to study prevalence and characteristics of resistance to antibiotics of staphylococcal isolates from the bulk-tank milk of sheep flocks across Greece, to assess possible associations of the presence of antibiotic resistance with the quality of milk in these flocks and to evaluate flock-related factors potentially associated with antibiotic resistance among these isolates. A cross-sectional study was performed in 325 sheep flocks in Greece. Bulk-tank milk samples were collected for bacteriological examination; staphylococcal isolates were evaluated for resistance to 20 antibiotics. Oxacillin-resistant staphylococcal isolates, isolates resistant to any antibiotic, and multi-resistant isolates were recovered from 8.0%, 30.5%, and 12.0% of flocks, respectively. Of 232 isolates, 11.6% were resistant to oxacillin, 46.1% were resistant to at least one antibiotic, and 16.4% were multi-resistant. Resistance was seen more frequently among coagulase-negative (50.6%) than among (31.5%) isolates. Resistance was more frequent against penicillin and ampicillin (34.1% of isolates), clindamycin (17.7%), and fosfomycin (14.2%). An association was found between biofilm formation by staphylococci and resistance to fosfomycin. For recovery of oxacillin-resistant isolates, the lack of experience by farmers emerged as a significant factor; respective factors for the isolation of staphylococci resistant to any antibiotic or multi-resistant isolates were the early stage of the lactation period (0th-1st month) and the intensive management system applied in the flocks, respectively.
这项工作的目的是研究希腊各地羊群的散装罐牛奶中葡萄球菌分离株对抗生素的耐药性流行情况和特征，评估抗生素耐药性的存在与这些羊群牛奶质量之间的可能关联，并评估与这些分离株中抗生素耐药性潜在相关的羊群相关因素。在希腊的325个羊群中进行了一项横断面研究。采集散装罐牛奶样本进行细菌学检查；对葡萄球菌分离株进行20种抗生素的耐药性评估。分别从8.0%、30.5%和12.0%的羊群中分离出耐氧西林葡萄球菌分离株、对任何抗生素耐药的分离株和多重耐药分离株。在232株分离株中，11.6%对氧西林耐药，46.1%对至少一种抗生素耐药，16.4%为多重耐药。凝固酶阴性分离株（50.6%）的耐药性比凝固酶阳性分离株（31.5%）更常见。对青霉素和氨苄西林（34.1%的分离株）、克林霉素（17.7%）和磷霉素（14.2%）的耐药性更常见。发现葡萄球菌形成生物膜与对磷霉素的耐药性之间存在关联。对于耐氧西林分离株的检出，农民缺乏经验是一个重要因素；分离出对任何抗生素耐药的葡萄球菌或多重耐药分离株的相应因素分别是泌乳期早期（第0 - 1个月）和羊群采用的集约化管理系统。
