College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China.
Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Hohhot 010018, China.
J Vet Sci. 2021 Nov;22(6):e66. doi: 10.4142/jvs.2021.22.e66. Epub 2021 Aug 8.
Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide.
In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated.
Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis.
A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%-86.8% and 67.7%-75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5).
The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.
梅迪/维斯纳病毒（MVV）是一种传染性病毒病原体，给全球的绵羊产业造成了巨大的经济损失。
在中国，已在多个地区检测到 MVV，但对其分子特征和遗传变异的研究还不够深入。
因此，本研究通过对从中国西北地区获得的 MVV 株进行下一代测序，通过系统进化分析揭示其遗传进化。
鉴定出一株来自中国内蒙古（NM）的 MVV 株。序列分析表明，其全基因组长度为 9193bp。NM 株与参考株核苷酸的同源性比较显示，gag 和 env 的序列同源性分别为 77.1%-86.8%和 67.7%-75.5%。系统进化分析显示，NM 株与来自美洲的参考株密切相关，属于 A2 型。值得注意的是，在表面糖蛋白（SU5）的 C 末端可变区 4 有 5 个氨基酸插入和一个高度可变的基序。
本研究首次展示了从中国获得的 MVV 的全基因组序列。详细的分析为了解 MVV 的遗传特征提供了重要信息，研究结果丰富了 MVV 文库。
