Research Department, Guangxi Medical University Cancer Hospital, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China.
Department of Radiotherapy, Guangxi Medical University Cancer Hospital, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China.
Exp Biol Med (Maywood). 2022 Mar;247(5):395-408. doi: 10.1177/15353702211049149. Epub 2021 Nov 7.
In our studies, cyclin B1 () mRNA and protein were overexpressed in hepatocellular carcinoma (HCC) tissues compared with non-HCC tissues. Moreover, was overexpressed in the serum of HCC patients. The expression of was associated with several crucial clinicopathologic characteristics, and the HCC patients with overexpressed had worse overall survival outcomes. In the screening of interactional genes, a total of 266 upregulated co-expression genes, which were positively associated with , were selected from the datasets, and 67 downregulated co-expression genes, which were negatively associated with , were identified. The key genes might be functionally enriched in DNA replication and the cell cycle pathways. , , , and were selected for further research because they were highly connected in the protein-protein interaction networks. Upregulated , , and and downregulated might result in undesirable overall survival outcomes for HCC patients. The univariate Cox analysis results showed that and might be two independent risk factors, while might be protective in HCC. Therefore, may participate in the cell cycle of HCC by regulating DNA replication, and may provide a direction for the diagnosis of early-stage HCC and targeted HCC therapy.
在我们的研究中，与非 HCC 组织相比，肝癌 (HCC) 组织中 cyclin B1 () mRNA 和蛋白表达过度。此外，在 HCC 患者的血清中也过度表达了。的表达与多个关键的临床病理特征相关，并且表达过度的 HCC 患者的总生存结局更差。在相互作用基因的筛选中，从数据集中共选择了 266 个与 正相关的上调共表达基因，鉴定了 67 个与 负相关的下调共表达基因。关键基因可能在 DNA 复制和细胞周期途径中具有功能富集。因为它们在蛋白质-蛋白质相互作用网络中高度连接，所以选择 、 、 和 进行进一步研究。上调的 和 以及下调的 可能导致 HCC 患者的总生存结果不佳。单因素 Cox 分析结果表明 和 可能是两个独立的危险因素，而 可能在 HCC 中具有保护作用。因此，可能通过调节 DNA 复制参与 HCC 的细胞周期，而 可能为早期 HCC 的诊断和 HCC 的靶向治疗提供方向。
