Jerez Puebla Luis Enrique, Núñez Fernández Fidel A, Fraga Nodarse Jorge, Cordovi Prado Raul, Atencio Millán Iraís, Cruz Rodríguez Iredys, Fimia Duarte Rigoberto, Sánchez Romero Marina Del Carmen, Ortega Medina Sahily de la Caridad, Del Risco Ubaldo, Pérez Santos Lissette, Robertson Lucy J
Departamento de Parasitología, Instituto "Pedro Kourí", Autopista Novia del Mediodía Km 6½ e/Autopista Nacional y Carretera Central, La Habana, Cuba.
Latin-American School of Medical Science, La Habana, Cuba.
Food Waterborne Parasitol. 2021 Oct 15;25:e00136. doi: 10.1016/j.fawpar.2021.e00136. eCollection 2021 Dec.
Taeniosis is a neglected disease, particularly in developing countries, and is caused by infection with the adult tapeworm of either , and . Of these, is of primary concern due to the potential for cysticercosis should eggs be ingested. In Cuba, all cases of taeniosis are assumed to be caused by , although some cases of cysticercosis have been documented. It is therefore important to gain further insights regarding the species causing taeniosis in Cuba, especially as diagnostic records indicate an increasing incidence, with the highest number of cases reported in 2020. In this study, we analysed 37 -positive faecal samples (or proglottids isolated from faecal samples) from the period 2001 until 2020 from all regions of the country. Genomic DNA was extracted from the samples, which had been stored in 10% formalin, using the QIAamp Tissue Kit. Species identification was carried out by duplex real-time PCR targeting the mitochondrial DNA. All cases were found to be and sequence analysis of three isolates confirmed the identification of this species. Our data do not provide any evidence that currently occurs in Cuba. However, given the relatively low number of samples analysed here, that the parasite may be imported with visitors or travellers who have been in endemic countries, and that taeniosis has relatively mild symptoms and thus infected patients may not seek medical attention, we recommend species determination for all taeniosis cases reported in Cuba.
绦虫病是一种被忽视的疾病，在发展中国家尤为如此，它是由感染牛带绦虫、猪带绦虫或亚洲带绦虫的成虫引起的。其中，由于摄入猪带绦虫卵可能导致囊尾蚴病，猪带绦虫是主要关注对象。在古巴，所有绦虫病病例都被认为是由牛带绦虫引起的，尽管也记录了一些囊尾蚴病病例。因此，进一步了解古巴引起绦虫病的物种非常重要，特别是鉴于诊断记录显示发病率在上升，2020年报告的病例数最多。在本研究中，我们分析了2001年至2020年期间从该国所有地区采集的37份牛带绦虫阳性粪便样本（或从粪便样本中分离出的节片）。使用QIAamp组织试剂盒从保存在10%福尔马林中的样本中提取基因组DNA。通过针对线粒体DNA的双重实时PCR进行物种鉴定。所有病例均被发现为牛带绦虫，对三个分离株的序列分析证实了该物种的鉴定。我们的数据没有提供任何证据表明猪带绦虫目前在古巴存在。然而，鉴于这里分析的样本数量相对较少，该寄生虫可能由去过流行国家的游客或旅行者带入，并且绦虫病症状相对较轻，因此感染患者可能不会寻求医疗帮助，我们建议对古巴报告的所有绦虫病病例进行物种鉴定。
