State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Molecules. 2021 Oct 27;26(21):6480. doi: 10.3390/molecules26216480.
The extracellular secreted protein of () plays an important role in maintaining the homeostasis of the human intestinal microenvironment. However, the mechanism(s) of interaction remain unclear. Lysozyme is a kind of antibacterial peptide. In this study, the amino acid sequence of a lysozyme-like protein of based on whole-genome data of an isolate from human gut feces was found. We further predicted functional domains from the amino acid sequence, purified the protein, and verified its bioactivity. The growth of some bacteria were significantly delayed by the 020402_LYZ M1 protein. In addition, the gut microbiota was analyzed via high-throughput sequencing of 16S rRNA genes and an in vitro fermentation model, and the fluctuations in the gut microbiota under the treatment of 020402_LYZ M1 protein were characterized. The 020402_LYZ M1 protein affected the composition of human gut microbiota significantly, implying that the protein is able to communicate with intestinal microbes as a regulatory factor.
（）的细胞外分泌蛋白在维持人体肠道微环境的动态平衡中发挥着重要作用。然而，其相互作用的机制尚不清楚。溶菌酶是一种抗菌肽。在本研究中，根据从人肠道粪便分离株的全基因组数据，发现了一种溶菌酶样蛋白的氨基酸序列。我们进一步从氨基酸序列预测了功能域，纯化了该蛋白，并验证了其生物活性。020402_LYZ M1 蛋白显著延迟了一些细菌的生长。此外，通过 16S rRNA 基因高通量测序和体外发酵模型分析了肠道微生物群，并对 020402_LYZ M1 蛋白处理下肠道微生物群的波动特征进行了描述。020402_LYZ M1 蛋白显著影响了人类肠道微生物群的组成，表明该蛋白作为一种调节因子能够与肠道微生物进行交流。
