State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Key Laboratory for Southwest Microbial Diversity of the Ministry of Education, and School of Life Science, Yunnan University, Kunming, China.
School of Resource, Environment and Chemistry, Chuxiong Normal University, Chuxiong, China.
J Appl Microbiol. 2022 Mar;132(3):2144-2156. doi: 10.1111/jam.15370. Epub 2021 Nov 24.
Phospholipase C (PLC) is a hydrolase involved in signal transduction in eukaryotic cells. This study aimed to understand the function of PLC in the nematode-trapping fungus Arthrobotrys oligospora.
Orthologous PLC (AoPLC2) of A. oligospora was functionally analysed using gene disruption and multi-phenotypic analysis. Disrupting Aoplc2 caused a deformation of partial hyphal cells (about 10%) and conidia (about 50%), decreased the number of nuclei in both conidia and hyphal cells, and increased the accumulation of lipid droplets. Meanwhile, the sporulation-related genes fluG and abaA were downregulated in ΔAoplc2 mutants than in the wild-type strain. Moreover, ΔAoplc2 mutants were more sensitive to osmotic stressors. Importantly, the number of traps, electron-dense bodies in traps, and nematicidal activity of ΔAoplc2 mutants were reduced, and the shape of the traps was deformed. In addition, AoPLC2 was involved in the biosynthesis of secondary metabolites in A. oligospora.
AoPLC2 plays an important role in the development of hyphae, spores, and cell nuclei, responses to stress, formation of traps, and predation of nematodes in A. oligospora.
This study reveals the various functions of phospholipase C and elucidates the regulation of trap morphogenesis in nematode-trapping fungi.
磷脂酶 C（PLC）是一种参与真核细胞信号转导的水解酶。本研究旨在了解线虫诱捕真菌节丛孢菌中 PLC 的功能。
通过基因敲除和多表型分析，对节丛孢菌的同源 PLC（AoPLC2）进行了功能分析。破坏 Aoplc2 会导致部分菌丝细胞（约 10%）和分生孢子（约 50%）变形，减少分生孢子和菌丝细胞中的核数，并增加脂滴的积累。同时，ΔAoplc2 突变体中与孢子形成相关的基因 fluG 和 abaA 的表达水平低于野生型菌株。此外，ΔAoplc2 突变体对渗透胁迫更敏感。重要的是，ΔAoplc2 突变体的诱捕器数量、诱捕器中的电子致密体和杀线虫活性减少，并且诱捕器的形状变形。此外，AoPLC2 参与了节丛孢菌中次生代谢物的生物合成。
AoPLC2 在节丛孢菌的菌丝、孢子和细胞核发育、应激反应、诱捕器形成和捕食线虫中发挥重要作用。
本研究揭示了磷脂酶 C 的多种功能，并阐明了线虫诱捕真菌中诱捕器形态发生的调控。
