儿童败血症的罕见病因。
rare cause of septicemia in children.
作者信息
T S Sreelekshmi, Ninan Marilyn M, Premanand Arul, Chacko Anila, Sahni Rani Diana, Michael Joy S
机构信息
Department of Clinical Microbiology, Christian Medical College, Vellore - 632004, India.
Department of Child Health, Christian Medical College, Vellore - 632004, India.
出版信息
Access Microbiol. 2021 Oct 11;3(10):000281. doi: 10.1099/acmi.0.000281. eCollection 2021.
UNLABELLED
is an emerging fungal pathogen in blood The main aim of this study was to describe the prevalence, methods of speciation and antifungal susceptibility of at a tertiary care centre.
METHODS
This was a retrospective study carried out at a tertiary care centre in South India. Over a period of 1 year, three were isolated from blood culture identified by MALDI-TOF MS Version 3.2 and were confirmed by ITS sequencing. Susceptibility testing was carried out by micro broth dilution.
RESULTS
All three patients had a common risk factor of prolonged ICU stay but the source of infection could not be identified. isolates were identified by MALDI-TOF and confirmed by ITS sequencing. They were pansusceptible to all tested antifungal drugs. Among these, two patients who were treated in hospital had good clinical outcome and response to antifungal drugs. A third patient was lost to follow up.
CONCLUSION
was predominantly seen between 0-3 month olds. Conventional methods of speciation were unable to identify to species level. Rapid identification was done by MALDI-TOF MS and confirmed by sequencing. Rapid identification leads to prompt treatment and favours a good clinical outcome.
未标记
是一种血液中新兴的真菌病原体。本研究的主要目的是描述在一家三级医疗中心的患病率、物种鉴定方法和抗真菌药敏性。
方法
这是一项在印度南部一家三级医疗中心进行的回顾性研究。在1年的时间里，从血培养中分离出3株，通过MALDI-TOF MS 3.2版进行鉴定，并通过ITS测序进行确认。药敏试验通过微量肉汤稀释法进行。
结果
所有3例患者都有长时间入住重症监护病房的共同危险因素，但感染源无法确定。通过MALDI-TOF鉴定分离株，并通过ITS测序进行确认。它们对所有测试的抗真菌药物均敏感。其中，2例在医院接受治疗的患者临床结局良好，对抗真菌药物有反应。第3例患者失访。
结论
主要见于0至3个月大的婴儿。传统的物种鉴定方法无法将鉴定到物种水平。通过MALDI-TOF MS进行快速鉴定，并通过测序进行确认。快速鉴定可导致及时治疗，并有利于良好的临床结局。
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