采用选择离子流管质谱法（SIFT-MS）对呼出气中的挥发性代谢物进行定量分析。
Quantification of volatile metabolites in exhaled breath by selected ion flow tube mass spectrometry, SIFT-MS.
作者信息
Španěl Patrik, Smith David
机构信息
J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejškova 3, 182 23 Prague 8, Czech Republic.
出版信息
Clin Mass Spectrom. 2020 Feb 13;16:18-24. doi: 10.1016/j.clinms.2020.02.001. eCollection 2020 Apr.
Abstract
Selected ion flow tube mass spectrometry, SIFT-MS, is a non-separative method for direct quantitative analyses of volatile compounds, VOCs, in air and humid breath based on chemical ionization. Selected reagent ions, either HO, NO or O (non-reactive with major components of air), ionize analyte molecules during a defined time in a flow tube by ion-molecule reactions thus producing analyte ions that are characteristic of the neutral analyte VOCs. Concentrations can be calculated in real-time from the ion count rates. Direct on-line analysis of single or multiple breath exhalations or off-line analysis of breath samples collected into bags can be performed. Several volatile breath metabolites have been quantified by SIFT-MS, including ammonia, acetone, hydrogen cyanide, alcohols, pentane, acetic acid, methane, and sulphur compounds. Their potential as biomarkers is discussed.
摘要
选择离子流管质谱法（SIFT-MS）是一种基于化学电离对空气和潮湿呼吸气中的挥发性化合物（VOCs）进行直接定量分析的非分离方法。选择的试剂离子，即HO、NO或O （与空气的主要成分不发生反应），在流动管中通过离子-分子反应在规定时间内使分析物分子电离，从而产生中性分析物VOCs特有的分析物离子。浓度可根据离子计数率实时计算。可以对单次或多次呼气进行直接在线分析，也可以对收集到袋子中的呼气样本进行离线分析。几种挥发性呼吸代谢物已通过SIFT-MS进行了定量，包括氨、丙酮、氰化氢、醇类、戊烷、乙酸、甲烷和硫化合物。文中讨论了它们作为生物标志物的潜力。
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