Department of Chemical Engineering, Fajar University, Makassar 90231, Indonesia.
Research Center for Brackish Aquaculture Fisheries and Extension Fisheries, Maros 90512, Indonesia.
Molecules. 2021 Nov 13;26(22):6851. doi: 10.3390/molecules26226851.
Every petroleum-processing plant produces sewage sludge containing several types of polycyclic aromatic hydrocarbons (PAHs). The degradation of PAHs via physical, biological, and chemical methods is not yet efficient. Among biological methods, the use of marine sponge symbiont bacteria is considered an alternative and promising approach in the degradation of and reduction in PAHs. This study aimed to explore the potential performance of a consortium of sponge symbiont bacteria in degrading anthracene and pyrene. Three bacterial species ( strain GLB197, strain SLG510A3-8, and strain SLCDA 976) were mixed to form the consortium. The interaction between the bacterial consortium suspension and PAH components was measured at 5 day intervals for 25 days. The biodegradation performance of bacteria on PAH samples was determined on the basis of five biodegradation parameters. The analysis results showed a decrease in the concentration of anthracene (21.89%) and pyrene (7.71%), equivalent to a ratio of 3:1, followed by a decrease in the abundance of anthracene (60.30%) and pyrene (27.52%), equivalent to a ratio of 2:1. The level of pyrene degradation was lower than that of the anthracene due to fact that pyrene is more toxic and has a more stable molecular structure, which hinders its metabolism by bacterial cells. The products from the biodegradation of the two PAHs are alcohols, aldehydes, carboxylic acids, and a small proportion of aromatic hydrocarbon components.
每个石油加工工厂都会产生含有多种多环芳烃（PAHs）的污水污泥。通过物理、生物和化学方法降解 PAHs 的效果还不够理想。在生物方法中，利用海洋海绵共生细菌被认为是一种替代的、有前途的方法，可以降解和减少 PAHs。本研究旨在探索海绵共生细菌混合物在降解蒽和芘方面的潜在性能。三种细菌（菌株 GLB197、菌株 SLG510A3-8 和菌株 SLCDA 976）混合形成了混合物。在 25 天的时间里，每隔 5 天测量一次细菌混合物悬浮液与 PAH 成分之间的相互作用。根据五个生物降解参数来确定细菌对 PAH 样品的生物降解性能。分析结果表明，蒽（21.89%）和芘（7.71%）的浓度降低，相当于 3:1 的比例，随后蒽（60.30%）和芘（27.52%）的丰度降低，相当于 2:1 的比例。由于芘毒性更高，分子结构更稳定，阻碍了细菌细胞对其的代谢，因此芘的降解水平低于蒽。这两种 PAHs 的生物降解产物是醇、醛、羧酸和一小部分芳烃成分。
