Natural Products Laboratory, Campus Darcy Ribeiro, School of Health Sciences, University of Brasilia, Brasília 70910-900, DF, Brazil.
Molecules. 2021 Nov 22;26(22):7062. doi: 10.3390/molecules26227062.
The purpose of this systematic review was to identify the available literature of production, purification, and characterization of proteases by endophytic fungi. There are few complete studies that entirely exhibit the production, characterization, and purification of proteases from endophytic fungi. This study followed the PRISMA, and the search was conducted on five databases: PubMed, PMC, Science Direct, Scopus Articles, and Web of Science up until 18 May 2021, with no time or language restrictions. The methodology of the selected studies was evaluated using GRADE. Protease production, optimization, purification, and characterization were the main evaluated outcomes. Of the 5540 initially gathered studies, 15 met the inclusion criteria after a two-step selection process. Only two studies optimized the protease production using statistical design and two reported enzyme purification and characterization. The genus and were the most cited among the eleven different genera of endophytic fungi evaluated in the selected articles. Six studies proved the ability of some endophytic fungi to produce fibrinolytic proteases, demonstrating that endophytic fungi can be exploited for the further production of agents used in thrombolytic therapy. However, further characterization and physicochemical studies are required to evaluate the real potential of endophytic fungi as sources of industrial enzymes.
本系统评价的目的是确定可获得的内生真菌产生、纯化和鉴定蛋白酶的文献。仅有少数完整的研究完全展示了内生真菌来源的蛋白酶的生产、特性和纯化。本研究遵循 PRISMA 原则，于 2021 年 5 月 18 日之前在五个数据库（PubMed、PMC、Science Direct、Scopus Articles 和 Web of Science）上进行了检索，没有时间或语言限制。使用 GRADE 评估了所选研究的方法学。蛋白酶的生产、优化、纯化和鉴定是主要的评估结果。在最初收集的 5540 项研究中，经过两步筛选过程，有 15 项符合纳入标准。只有两项研究使用统计设计优化了蛋白酶的生产，有两项报告了酶的纯化和特性。在所选择的文章中评估的十一种不同内生真菌属中， 和 被引用最多。六项研究证明了一些内生真菌产生纤维蛋白溶酶的能力，表明内生真菌可用于进一步生产溶栓治疗中使用的制剂。然而，还需要进一步的特性和物理化学研究来评估内生真菌作为工业酶源的真正潜力。
