酿酒酵母5.8S核糖体RNA基因的定位
Location of the 5.8S rRNA gene of Saccharomyces cerevisiae.
作者信息
Skryabin K G, Maxam A M, Petes T D, Hereford L
出版信息
J Bacteriol. 1978 Apr;134(1):306-9. doi: 10.1128/jb.134.1.306-309.1978.
Abstract
Direct DNA sequence analysis of Saccharomyces cerevisiae ribosomal DNA cloned in an Escherichia coli plasmid revealed part of the structural gene for 5.8S rRNA at one end of a 700-base-pair EcoRI fragment. Taken with the previously established EcoRI restriction map of the ribosomal repeat unit, this sequence establishes that the yeast 5.8S RNA segment is located between the 18S and 28S segments in the 42S rRNA precursor and in the DNA which codes for it.
摘要
对克隆于大肠杆菌质粒中的酿酒酵母核糖体DNA进行直接DNA序列分析，结果显示在一个700碱基对的EcoRI片段一端存在5.8S rRNA的部分结构基因。结合先前建立的核糖体重复单元的EcoRI限制酶切图谱，该序列确定酵母5.8S RNA片段位于42S rRNA前体及其编码DNA中的18S和28S片段之间。
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