College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Jiangsu Key Laboratory of Artificial Functional Materials, Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing 210023, China.
Shenzhen Research Institute of Nanjing University, Shenzhen 518000, China.
Int J Mol Sci. 2021 Nov 29;22(23):12911. doi: 10.3390/ijms222312911.
As an important component that constitutes all the cells and tissues of the human body, protein is involved in most of the biological processes. Inspired by natural protein systems, considerable efforts covering many discipline fields were made to design artificial protein assemblies and put them into application in recent decades. The rapid development of structural DNA nanotechnology offers significant means for protein assemblies and promotes their application. Owing to the programmability, addressability and accurate recognition ability of DNA, many protein assemblies with unprecedented structures and improved functions have been successfully fabricated, consequently creating many brand-new researching fields. In this review, we briefly introduced the DNA-based protein assemblies, and highlighted the limitations in application process and corresponding strategies in four aspects, including biological catalysis, protein detection, biomedicine treatment and other applications.
作为构成人体所有细胞和组织的重要组成部分，蛋白质参与了大多数的生物过程。受天然蛋白质体系的启发，近几十年来，涵盖多个学科领域的大量工作致力于设计人工蛋白质组装体并将其应用于实际。结构 DNA 纳米技术的快速发展为蛋白质组装体提供了重要的手段，并推动了它们的应用。由于 DNA 的可编程性、可寻址性和精确识别能力，许多具有前所未有的结构和改进功能的蛋白质组装体已经被成功制造出来，从而创造了许多全新的研究领域。在这篇综述中，我们简要介绍了基于 DNA 的蛋白质组装体，并从生物催化、蛋白质检测、生物医药治疗和其他应用等四个方面重点强调了在应用过程中的局限性及相应的策略。
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