McGahan M C, Fleisher L N
Curr Eye Res. 1986 Sep;5(9):641-5. doi: 10.3109/02713688609015131.
The effects of aqueous and vitreous humors and plasma on the rate of auto-oxidation of a rabbit brain homogenate were measured. Both aqueous and vitreous humors from normal eyes increased, while plasma decreased the rate of oxidation in the homogenate. During endotoxin-induced ocular inflammation the copper (Cu) and iron (Fe) concentrations of both the aqueous and vitreous humors increased, most likely due to the influx of their plasma binding proteins, ceruloplasmin (Cu) and transferrin (Fe). As both proteins are known to be antioxidants, it was not surprising to find that the aqueous and vitreous humor from the inflamed eyes had significant antioxidant activity. This antioxidant activity correlated well with the concentrations of Cu and Fe in aqueous humor and Cu but not Fe in the vitreous humor throughout the time course of the inflammatory response. Thus, entry of plasma proteins through disrupted blood ocular barriers may function in protecting ocular tissues against the increased oxidation which occurs during inflammation.
测量了房水、玻璃体和血浆对兔脑匀浆自氧化速率的影响。正常眼的房水和玻璃体均能提高匀浆的氧化速率，而血浆则降低其氧化速率。在内毒素诱导的眼部炎症过程中，房水和玻璃体中的铜（Cu）和铁（Fe）浓度均升高，这很可能是由于它们的血浆结合蛋白，即铜蓝蛋白（Cu）和转铁蛋白（Fe）的流入所致。由于已知这两种蛋白都是抗氧化剂，因此发现炎症眼的房水和玻璃体具有显著的抗氧化活性也就不足为奇了。在整个炎症反应过程中，这种抗氧化活性与房水中Cu和Fe的浓度以及玻璃体中Cu而非Fe的浓度密切相关。因此，血浆蛋白通过受损的血眼屏障进入眼内，可能起到保护眼组织免受炎症期间氧化增加的作用。
