Genome-Wide Identification and Expression Analysis of the Plant U-Box Protein Gene Family in .

The gene encodes a ubiquitin ligase that contains a U-box domain. The plant U-box (PUB) protein plays an important role in the plant stress response; however, very few studies have investigated the role of these proteins in Moso bamboo (). Thus, more research on PUB proteins is necessary to understand the mechanisms of stress tolerance in . In this study, we identified 121 members of the PUB family in (), using bioinformatics based on the V2 genome build. The genes of showed an uneven distribution among the chromosomes. Phylogenetic analysis of the genes between and suggested that these genes can be classified into eight subgroups (Groups I-VIII) based on their structural and phylogenetic features. All genes and the structure of their encoded proteins were identified in . We further investigated the expression pattern of genes in different tissues, including the leaves, panicles, rhizomes, roots, and shoots. The qRT-PCR results showed that expression of three genes, , and , was upregulated at low temperatures compared to that at 25°C. The expression levels of two , and , were upregulated under drought stress. These results suggest that the genes play an important role in resistance to low temperatures and drought in . This research provides new insight into the function, diversity, and characterization of genes in and provides a basis for understanding their biological roles and molecular mechanisms.