Division of the National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA.
Battelle, Columbus, OH, USA.
Food Chem Toxicol. 2022 Feb;160:112769. doi: 10.1016/j.fct.2021.112769. Epub 2021 Dec 18.
Black cohosh (Actaea racemosa L.) is a botanical supplement marketed to women of all ages. Due to paucity of data to assess the safe use, the National Toxicology Program (NTP) is evaluating the toxicity of black cohosh. The use of an authentic, quality material is imperative to generate robust data. Because botanical materials are complex mixtures with variable composition, the selection of a material is challenging. We describe selection and phytochemical characterization of an unformulated black cohosh root extract (i.e., an extract that serves as source material for a formulated product) to be used in the NTP assessments. A material was selected using a combination of non-targeted and targeted chemical analyses, including confirmation of authenticity, absence of contaminants and adulterants, and similarity to a popular black cohosh product used by consumers. Thirty-nine constituents covering three major classes, triterpene glycosides, phenolic acids, and alkaloids were identified. Among constituents quantified, triterpene glycosides made up approximately 4.7% (w/w) with total constituents quantified making up 5.8% (w/w) of the extract. Non-targeted chemical analysis followed by chemometric analysis of various materials sold as black cohosh, and reference materials for black cohosh and other Actaea species further confirmed the suitability of the selected extract for use.
黑升麻（Actaea racemosa L.）是一种植物补充剂，面向所有年龄段的女性销售。由于缺乏评估安全使用的相关数据，国家毒理学计划（NTP）正在评估黑升麻的毒性。使用真实、高质量的材料对于生成稳健的数据至关重要。由于植物材料是具有可变成分的复杂混合物，因此选择材料具有挑战性。我们描述了一种未配方的黑升麻根提取物（即作为配方产品原料的提取物）的选择和植物化学特征，将用于 NTP 评估。该材料是使用非靶向和靶向化学分析的组合选择的，包括真实性确认、无污染物和掺杂物以及与消费者使用的流行黑升麻产品相似。鉴定出 39 种成分，涵盖了三个主要类别，三萜糖苷、酚酸和生物碱。在所定量的成分中，三萜糖苷约占 4.7%（w/w），定量的总成分占提取物的 5.8%（w/w）。对各种作为黑升麻出售的材料以及黑升麻和其他 Actaea 物种的参考材料进行非靶向化学分析，并进行化学计量学分析，进一步证实了所选提取物的适用性。
