Nishida T, Nishino N, Takano M, Kawai K, Bando K, Masui Y, Nakai S, Hirai Y
Biochem Biophys Res Commun. 1987 Feb 27;143(1):345-52. doi: 10.1016/0006-291x(87)90671-1.
Clones of cDNAs encoding growth inhibitory factors for human melanoma cell line A375 were isolated from cDNA library prepared by using mRNA derived from human histiocytic lymphoma cell line U937 induced with PMA and further stimulated with LPS. Cloning was achieved using Okayama-Berg cDNA expression vector system that permits expression of the inserted cDNA segments in mammalian cells. By assaying the transfected COS-1 cells supernatants and cell extracts, we isolated two distinct cDNA clones encoding growth inhibitory factors. It was determined by the nucleotide sequences of the inserts, the cDNAs corresponded to IL-1 alpha and -1 beta. Our results indicate U937 cells can be induced to produce both interleukin-1s.
从使用经佛波酯（PMA）诱导并用脂多糖（LPS）进一步刺激的人组织细胞淋巴瘤细胞系U937来源的mRNA构建的cDNA文库中，分离出编码人黑色素瘤细胞系A375生长抑制因子的cDNA克隆。使用冈山-伯格cDNA表达载体系统进行克隆，该系统允许插入的cDNA片段在哺乳动物细胞中表达。通过检测转染的COS-1细胞的上清液和细胞提取物，我们分离出两个编码生长抑制因子的不同cDNA克隆。通过插入片段的核苷酸序列确定，这些cDNA分别对应于白细胞介素-1α和-1β。我们的结果表明，U937细胞可被诱导产生这两种白细胞介素-1。
