Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea.
Department of Pharmacology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea.
Nutrients. 2021 Dec 13;13(12):4448. doi: 10.3390/nu13124448.
In this study, we investigated the pharmacological effect of a water extract of Raphani Semen (RSWE) on alcoholic fatty liver disease (AFLD) using ethanol-induced AFLD mice (the NIAAA model) and palmitic acid (PA)-induced steatosis HepG2 cells. An RSWE supplement improved serum and hepatic triglyceride (TG) levels of AFLD mice, as well as their liver histological structure. To explore the molecular action of RSWE in the improvement of AFLD, we investigated the effect of RSWE on four major pathways for lipid homeostasis in the liver: free fatty acid transport, lipogenesis, lipolysis, and β-oxidation. Importantly, RSWE decreased the mRNA expression of de novo lipogenesis-related genes, such as Srebf1, Cebpa, Pparg, and Lpin1, as well as the protein levels of these factors, in the liver of AFLD mice. That these actions of RSWE affect lipogenesis was confirmed using PA-induced steatosis HepG2 cells. Overall, our findings suggest that RSWE has the potential for improvement of AFLD by inhibiting de novo lipogenesis.
在这项研究中，我们使用乙醇诱导的非酒精性脂肪性肝病（AFLD）小鼠（NIAAA 模型）和棕榈酸（PA）诱导的脂肪变性 HepG2 细胞，研究了萝卜籽水提取物（RSWE）对酒精性脂肪肝疾病（AFLD）的药理作用。RSWE 补充剂可改善 AFLD 小鼠的血清和肝脏甘油三酯（TG）水平，以及其肝脏组织学结构。为了探讨 RSWE 改善 AFLD 的分子作用机制，我们研究了 RSWE 对肝脏中四条主要脂质稳态途径的影响：游离脂肪酸转运、脂肪生成、脂肪分解和β-氧化。重要的是，RSWE 降低了 AFLD 小鼠肝脏中与从头脂肪生成相关的基因（如 Srebf1、Cebpa、Pparg 和 Lpin1）的 mRNA 表达以及这些因素的蛋白水平。用 PA 诱导的脂肪变性 HepG2 细胞证实了 RSWE 的这些作用影响脂肪生成。总的来说，我们的研究结果表明，RSWE 通过抑制从头脂肪生成具有改善 AFLD 的潜力。
