Division of Biological Science and Technology, Yonsei University, Wonju 26493, Korea.
Department of Analysis and Assessment, Mine Reclamation Corporation, Wonju 26464, Korea.
Viruses. 2021 Dec 17;13(12):2533. doi: 10.3390/v13122533.
The COVID-19 pandemic has resulted in a huge number of deaths from 2020 to 2021; however, effective antiviral drugs against SARS-CoV-2 are currently under development. Recent studies have demonstrated that green tea polyphenols, particularly EGCG, inhibit coronavirus enzymes as well as coronavirus replication in vitro. Herein, we examined the inhibitory effect of green tea polyphenols on coronavirus replication in a mouse model. We used epigallocatechin gallate (EGCG) and green tea polyphenols containing more than 60% catechin (GTP60) and human coronavirus OC43 (HCoV-OC43) as a surrogate for SARS-CoV-2. Scanning electron microscopy analysis results showed that HCoV-OC43 infection resulted in virion particle production in infected cells. EGCG and GTP60 treatment reduced coronavirus protein and virus production in the cells. Finally, EGCG- and GTP60-fed mice exhibited reduced levels of coronavirus RNA in mouse lungs. These results demonstrate that green tea polyphenol treatment is effective in decreasing the level of coronavirus in vivo.
从 2020 年到 2021 年，COVID-19 大流行导致了大量死亡；然而，目前正在开发针对 SARS-CoV-2 的有效抗病毒药物。最近的研究表明，绿茶多酚，特别是 EGCG，可抑制冠状病毒酶以及冠状病毒在体外的复制。在此，我们在小鼠模型中研究了绿茶多酚对冠状病毒复制的抑制作用。我们使用表没食子儿茶素没食子酸酯（EGCG）和含有超过 60%儿茶素的绿茶多酚（GTP60）以及人冠状病毒 OC43（HCoV-OC43）作为 SARS-CoV-2 的替代物。扫描电子显微镜分析结果表明，HCoV-OC43 感染导致感染细胞中产生病毒粒子。EGCG 和 GTP60 处理可减少细胞中的冠状病毒蛋白和病毒产量。最后，用 EGCG 和 GTP60 喂养的小鼠肺中冠状病毒 RNA 水平降低。这些结果表明，绿茶多酚治疗可有效降低体内冠状病毒水平。
