The State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China.
Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong, People's Republic of China.
ACS Sens. 2022 Jan 28;7(1):3-20. doi: 10.1021/acssensors.1c01647. Epub 2022 Jan 6.
DNA nanotweezers (DTs) are reversible DNA nanodevices that can optionally switch between opened and closed states. Due to their excellent flexibility and high programmability, they have been recognized as a promising platform for constructing a diversity of biosensors and logic gates, as well as a versatile tool for molecular biology studies. In this review, we provide an overview of biosensing applications using DTs. First, the design and working principle of DTs are introduced. Next, the signal producing principles of DTs are summarized. Furthermore, biosensing applications of DTs for varying targets and purposes, both in buffers and complex biological environments, are highlighted. Finally, we provide potential opportunities and challenges for the further development of DTs.
DNA 纳米钳（DTs）是一种可在打开和关闭状态之间任意切换的可逆 DNA 纳米器件。由于其优异的灵活性和高度的可编程性，它们已被认为是构建各种生物传感器和逻辑门的有前途的平台，以及用于分子生物学研究的多功能工具。在本综述中，我们介绍了使用 DTs 的生物传感应用。首先，介绍了 DTs 的设计和工作原理。接下来，总结了 DTs 的信号产生原理。此外，还强调了 DTs 在缓冲液和复杂生物环境中针对不同目标和用途的生物传感应用。最后，我们为 DTs 的进一步发展提供了潜在的机遇和挑战。
