Department of Pharmacy, The First People's Hospital of Yongkang Affiliated To Hangzhou Medical College, Yongkang, 321300, China.
School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China.
J Nat Med. 2022 Mar;76(2):468-475. doi: 10.1007/s11418-021-01588-w. Epub 2022 Jan 7.
Eight pentacyclic triterpenoids including two new ones (1, 2) were isolated from the fruits of Xanthium strumarium. Their structures were elucidated by extensive spectroscopic analysis. All isolates were evaluated for in vitro cytotoxic activity on HepG2, A549, HCT116 and SW480 cancer cells. Among them, the new compound 2 was found to exhibit significant cytotoxic activity on A549, HCT116 and SW480 cancer cells with IC values of 9.68, 4.27 and 7.58 μM, respectively. Further, 2 was selected for cell cycle analysis and results revealed that 2 could cause HCT116 cell cycle arrest in G1 phase. In addition, Annexin V-FITC/PI staining assay showed that 2 could induce the death of HCT116 cells.
从苍耳果实中分离得到 8 种五环三萜类化合物，包括 2 种新化合物（1、2）。通过广泛的光谱分析阐明了它们的结构。所有分离物均在 HepG2、A549、HCT116 和 SW480 癌细胞的体外细胞毒性活性方面进行了评估。其中，新化合物 2 对 A549、HCT116 和 SW480 癌细胞表现出显著的细胞毒性活性，IC 值分别为 9.68、4.27 和 7.58 μM。此外，选择 2 进行细胞周期分析，结果表明 2 可使 HCT116 细胞周期阻滞在 G1 期。此外，Annexin V-FITC/PI 染色试验表明，2 可诱导 HCT116 细胞死亡。
