Syed Rahamat Unissa, Moni Sivakumar S, Lila Amr S Abu, Abdallah Marwa H, Abouzied Amr S, Banu Humera, Alreshidi Khetam Saad Mutni, Alrashidi Badriah Mansour Wadid, Hadi Mohd Abdul, El-Horany Hemat, Abdelwahab Siddig Ibrahim, Taha Manal Mohamed Elhassan
Department of Pharmaceutics, College of Pharmacy, University of Hail, Hail 81442, Saudi Arabia.
Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan 45142, Saudi Arabia.
Life (Basel). 2022 Oct 19;12(10):1641. doi: 10.3390/life12101641.
The objective of this study was to characterize the bioactive ingredients and antiulcer effects of Lactuca sativa leaves. Several bioactive chemicals were found in the cold methanolic extract of Lactuca sativa leaves after gas chromatography-mass spectrometry (GC-MS) research: 9,12-octadecadienoic acid (Z,Z)-, cyclononasiloxane, octadecamethyl-, n-hexadecanoic acid, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl, octadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, 9-octadecenamide, (Z)-, hexadecanoic acid, stigmasterol, benzothiazole, ethyl iso-allocholate, and octacosane. Distinct fingerprint regions in GCMS indicated the existence of bioactive compounds. The leaf powder of Lactuca sativa (LPL) demonstrated substantial antiulcer properties at 400 mg/kg, which was almost equivalent to the standard drug at 20 mg/kg. The cytokine network was efficiently regulated by reducing the production of proinflammatory cytokines such as IL-1β, IL-6, and TNF-α. The levels of caspase-3 and caspase-9 were also considerably lowered at p < 0.05 significant level.
本研究的目的是表征生菜叶的生物活性成分和抗溃疡作用。通过气相色谱-质谱联用（GC-MS）研究，在生菜叶的冷甲醇提取物中发现了几种生物活性化学物质：9,12-十八碳二烯酸（Z,Z）-、环壬硅氧烷、十八甲基-、正十六烷酸、十六烷酸、2-羟基-1-（羟甲基）乙酯、十八烷酸、2-羟基-1-（羟甲基）乙酯、9-十八碳烯酰胺（Z）-、十六烷酸、豆甾醇、苯并噻唑、异别胆酸乙酯和二十八烷。GC-MS中的不同指纹区域表明存在生物活性化合物。生菜叶粉末（LPL）在400mg/kg时表现出显著的抗溃疡特性，这几乎等同于20mg/kg的标准药物。通过减少促炎细胞因子如IL-1β、IL-6和TNF-α的产生，细胞因子网络得到有效调节。在p<0.05的显著水平下，caspase-3和caspase-9的水平也显著降低。
