Hariprasad R, Suganya T L, John Serin Sarah, Raj P Rajesh, Kuriakose Feby, Anoop V N
Full Time Specialist in Endodontics, Department of Dentistry, Employees State Insurance Corporation Hospital, Ezhukone, Kollam, Kerala, India.
Senior Lecturer, Department of Conservative Dentistry and Endodontics, Rajas Dental College, Dr MGR Medical University, Kavalkinaru, Tamilnadu, India.
J Pharm Bioallied Sci. 2021 Nov;13(Suppl 2):S1696-S1699. doi: 10.4103/jpbs.jpbs_358_21. Epub 2021 Nov 10.
Assessment of growth factors with three different platelet preparations namely platelet rich fibrin, platelet rich plasma and lyophilized platelet-An invitro study Abstract Enhancing wound healing with patients own blood, particularly the platelet preparations have gained more popularity recently. These platelets excrete large amount of growth factors and cytokines that are associated with the healing process and regeneration. Even though these platelet concentrates are widely used in various forms, the mechanism behind that remains unclear. Therefore, this study aims at investigating the levels of growth factors in three different platelet preparations namely platelet rich plasma (PRP), platelet rich fibrin (PRF) and lyophilized platelets. Materials and Methods Autologous blood for preparing the platelet preparations was obtained from healthy donors aged between 25 to 35 years. The samples were then divided into three experimental groups. The preparation of PRP was done with the addition of anticoagulant and the PRF is prepared without adding it. The platelet counts in the blood were analyzed and the growth factors were quantitatively measured using ELISA reader. The statistical analysis was performed by using the Chi square test. Results In the quantitative analysis of growth factors LPL showed significant increase of the liberation of growth factors compared to PRP and PRF. Conclusions With the various recent advances in technologies for preparing these platelet concentrates this can be widely used in clinical practice more accurate in the future.
三种不同血小板制剂（即富血小板纤维蛋白、富血小板血浆和冻干血小板）生长因子的评估——一项体外研究 摘要 利用患者自身血液促进伤口愈合，特别是血小板制剂最近越来越受欢迎。这些血小板分泌大量与愈合过程和再生相关的生长因子和细胞因子。尽管这些血小板浓缩物以各种形式被广泛使用，但其背后的机制仍不清楚。因此，本研究旨在调查三种不同血小板制剂，即富血小板血浆（PRP）、富血小板纤维蛋白（PRF）和冻干血小板中的生长因子水平。 材料与方法 用于制备血小板制剂的自体血取自年龄在25至35岁之间的健康供者。然后将样本分为三个实验组。PRP的制备添加了抗凝剂，而PRF的制备未添加抗凝剂。分析血液中的血小板计数，并使用酶标仪对生长因子进行定量测量。采用卡方检验进行统计分析。 结果 在生长因子的定量分析中，与PRP和PRF相比，LPL显示出生长因子释放的显著增加。 结论 随着制备这些血小板浓缩物的技术最近取得的各种进展，其在未来临床实践中可更广泛、更准确地应用。
