Baker-Zander S A, Lukehart S A
Infect Immun. 1983 Nov;42(2):634-8. doi: 10.1128/iai.42.2.634-638.1983.
Protein antigens of Treponema pallidum, Nichols strain, and Treponema pertenue, Gauthier strain, were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques. Treponemal proteins were solubilized in 1% sodium dodecyl sulfate, electrophoresed on 12.5% polyacrylamide gels, and either stained with Coomassie brilliant blue or electrophoretically transferred to nitrocellulose paper. These antigen blots were incubated with sera from rabbits infected with either T. pallidum or T. pertenue and 125I-labeled staphylococcal protein A and exposed to X-ray film for visualization of antigenic molecules. Protein profiles of each organism separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue showed no distinguishable differences. Antigenic profiles as determined by Western blots were similar with two exceptions. A 39,500-dalton band was present on T. pertenue but absent from T. pallidum, and a 19,000-dalton band was present on T. pallidum but absent from T. pertenue (although two additional antigenic bands at 21,000 and 18,000 daltons were seen on T. pertenue). Because these differences were detected by using antisera raised against either T. pallidum or T. pertenue, these molecules must contain some antigenic determinants in common despite their differences in molecular weight.
用十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳和蛋白质印迹技术鉴定了梅毒螺旋体Nichols株和雅司螺旋体Gauthier株的蛋白质抗原。将梅毒螺旋体蛋白溶解于1%十二烷基硫酸钠中，在12.5%聚丙烯酰胺凝胶上进行电泳，然后用考马斯亮蓝染色或电泳转移至硝酸纤维素纸上。将这些抗原印迹与感染梅毒螺旋体或雅司螺旋体的兔血清以及125I标记的葡萄球菌蛋白A一起温育，然后与X线胶片接触以观察抗原分子。经十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分离并用考马斯亮蓝染色后，每种微生物的蛋白质图谱没有明显差异。通过蛋白质印迹确定的抗原图谱相似，但有两个例外。雅司螺旋体上有一条39,500道尔顿的条带，而梅毒螺旋体上没有；梅毒螺旋体上有一条19,000道尔顿的条带，而雅司螺旋体上没有（尽管在雅司螺旋体上还可见到另外两条21,000和18,000道尔顿的抗原条带）。由于使用针对梅毒螺旋体或雅司螺旋体产生的抗血清检测到了这些差异，因此尽管这些分子分子量不同，但它们必定含有一些共同的抗原决定簇。
