Department of Medicine, Leon H. Charney Division of Cardiology (N.E., C.G.), New York University Grossman School of Medicine, NYU Langone Health' New York.
NYU Cardiovascular Research Center (N.E., C.G.), New York University Grossman School of Medicine, NYU Langone Health' New York.
Arterioscler Thromb Vasc Biol. 2022 Mar;42(3):243-252. doi: 10.1161/ATVBAHA.121.315849. Epub 2022 Feb 3.
The development of innovative single-cell technologies has allowed the high-dimensional transcriptomic and proteomic profiling of individual blood and tissue cells. Recent single-cell studies revealed a new cellular heterogeneity of atherosclerotic plaque tissue and allowed a better understanding of distinct immune functional states in the context of atherosclerosis. In this brief review, we describe how single-cell technologies have shed a new light on the cellular composition of atherosclerotic plaques, and their response to diet perturbations or genetic manipulation in mouse models of atherosclerosis. We discuss how single-cell RNA sequencing, cellular indexing of transcriptomes and epitopes by sequencing, transposase-accessible chromatin with high-throughput sequencing, and cytometry by time-of-flight platforms have empowered the identification of discrete immune, endothelial, and smooth muscle cell alterations in atherosclerosis progression and regression. Finally, we review how single-cell approaches have allowed mapping the cellular and molecular composition of human atherosclerotic plaques and the discovery of new immune alterations in plaques from patients with stroke.
单细胞技术的创新发展使得对单个血液和组织细胞进行高维转录组学和蛋白质组学分析成为可能。最近的单细胞研究揭示了动脉粥样硬化斑块组织中的一种新的细胞异质性，并使人们能够更好地理解动脉粥样硬化背景下不同的免疫功能状态。在这篇简短的综述中，我们描述了单细胞技术如何揭示动脉粥样硬化斑块的细胞组成，以及它们对饮食干扰或遗传操作的反应在动脉粥样硬化小鼠模型中的情况。我们讨论了单细胞 RNA 测序、通过测序对转录物和表位进行细胞标记、高通量测序的转座酶可及染色质，以及飞行时间平台的细胞术如何能够鉴定动脉粥样硬化进展和消退过程中离散的免疫、内皮和平滑肌细胞改变。最后，我们综述了单细胞方法如何能够描绘人类动脉粥样硬化斑块的细胞和分子组成，并发现中风患者斑块中的新免疫改变。
