Li Zhenming, Wu Dexin, Guo Yu, Mao Weili, Zhao Nan, Zhao Meirong, Jin Hangbiao
College of Chemical and Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
Hangzhou Xinjing Environmental Technology Co., Ltd., Hangzhou 310007, PR China.
Sci Total Environ. 2022 Jun 10;824:153792. doi: 10.1016/j.scitotenv.2022.153792. Epub 2022 Feb 10.
Presence of phthalate metabolites (PMs) in human serum has been well documented. However, the distribution pattern of PMs in different human blood matrixes remains not well known. To investigate this, paired serum and whole blood samples were collected from 145 adults (76 males and 69 females) in Quzhou, China, and analyzed for nine PMs in this study. All PMs had high detection frequencies (> 70%) in human serum and whole blood, except mono benzyl phthalate. Total concentrations of detected PMs in serum and whole blood were 0.70-61 ng/mL (mean 12 ng/mL) and 1.6-33 ng/mL (7.5 ng/mL), respectively. Mono methyl phthalate (MMP), mono (2-ethylhexyl) phthalate, and mono butyl phthalate were consistently the predominant PMs in human serum and whole blood, with the mean concentrations of 3.4 and 2.0 ng/mL, 3.3 and 2.1 ng/mL, and 2.8 and 1.8 ng/mL, respectively. Females had higher mean serum concentrations of PMs, except MBP, than males. Youngest age group (20-30 years) consistently had the lowest mean whole blood levels of all PMs. For the first time, the distribution pattern of PMs in human blood was evaluated based on the calculated partitioning coefficient (K) between serum and whole blood. MMP had the highest mean K value (1.6; 10th-90th percentile: 1.0-2.2), while mono (2-ethyl-5-oxohexyl) phthalate had the lowest mean K value (0.63; 10th-90th percentile: 0.25-1.3). These results help better understand the occurrence of PMs in human blood.
人体血清中邻苯二甲酸酯代谢物（PMs）的存在已有充分记录。然而，PMs在不同人体血液基质中的分布模式仍不太清楚。为了对此进行研究，本研究从中国衢州的145名成年人（76名男性和69名女性）中采集了配对的血清和全血样本，并分析了其中的9种PMs。除单苄基邻苯二甲酸酯外，所有PMs在人体血清和全血中的检测频率都很高（>70%）。血清和全血中检测到的PMs总浓度分别为0.70 - 61 ng/mL（平均12 ng/mL）和1.6 - 33 ng/mL（7.5 ng/mL）。单甲基邻苯二甲酸酯（MMP）、单（2-乙基己基）邻苯二甲酸酯和单丁基邻苯二甲酸酯一直是人体血清和全血中主要的PMs，其平均浓度分别为3.4和2.0 ng/mL、3.3和2.1 ng/mL、2.8和1.8 ng/mL。除单丁基邻苯二甲酸苄酯外，女性的PMs平均血清浓度高于男性。最年轻的年龄组（20 - 30岁）的所有PMs平均全血水平一直最低。本研究首次基于计算得到的血清和全血之间的分配系数（K）评估了PMs在人体血液中的分布模式。MMP的平均K值最高（1.6；第10 - 90百分位数：1.0 - 2.2），而单（2-乙基-5-氧代己基）邻苯二甲酸酯的平均K值最低（0.63；第10 - 90百分位数：0.25 - 1.3）。这些结果有助于更好地了解PMs在人体血液中的存在情况。
