Haneda K, Horiuchi T
Tohoku J Exp Med. 1986 Jan;148(1):49-56. doi: 10.1620/tjem.148.49.
A method of measuring total circulating blood volume (TBV) using indocyanine green (ICG) dye was investigated in 7 dogs and 16 patients. Since concentration of injected ICG decreases exponentially from the circulating system after recirculation, concentration of the dye at the time of appearance (Cta) can be extrapolated by extending the slope of the dye dilution curve transcribed to a semi-logarithmic graph. TBV was calculated using the following formula: TBV = I/Cta, where I is the amount (mg) of ICG injected. Repeat measurements of TBV using ICG demonstrated good reproducibility (average coefficient of variation, 2.0%) and when compared to measurements made with Evans blue dye resulted in a correlation coefficient of 0.98 or more. We conclude that assessment of TBV using ICG is reliable and experimentally and clinically feasible.
在7只犬和16名患者中研究了一种使用吲哚菁绿（ICG）染料测量总循环血量（TBV）的方法。由于再循环后注入的ICG浓度从循环系统呈指数下降，因此可以通过延长转录到半对数图上的染料稀释曲线的斜率来外推出现时染料的浓度（Cta）。使用以下公式计算TBV：TBV = I/Cta，其中I是注入的ICG量（mg）。使用ICG重复测量TBV显示出良好的可重复性（平均变异系数为2.0%），并且与使用伊文思蓝染料进行的测量相比，相关系数为0.98或更高。我们得出结论，使用ICG评估TBV是可靠的，在实验和临床上都是可行的。
