Aguree Sixtus, Gernand Alison D
Department of Nutritional Sciences, The Pennsylvania State University, 110 Chandlee Laboratory, University Park, PA, 16802, United States.
MethodsX. 2019 May 8;6:1072-1083. doi: 10.1016/j.mex.2019.05.003. eCollection 2019.
Plasma volume (PV) can be an important marker of health status and may affect the interpretation of plasma biomarkers, but is rarely measured due to the complexity and time required. Indocyanine green (ICG) is a water-soluble tricarbocyanine dye with a circulatory half-life of 2-3 min, allowing for quick clearance and repeated use. It is used extensively in medical diagnostic tests including ophthalmologic imaging, liver function, and cardiac output, particularly in critical care. ICG has been validated for measuring PV in humans, however previous work has provided minimal published details or has focused on a single aspect of the method. We aimed to develop a detailed, optimal protocol for the use of ICG to measure PV in women of reproductive age. We combined best practices from other studies and optimized the protocol for efficiency. •This method reduces the time from blood collection to PV determination to ˜2 h and the amount of plasma required to estimate PV to 2.5 mL (1.5 mL before ICG injection and 1.0 mL post-injection).•Participant inconvenience is reduced by inserting an intravenous (IV) catheter in only one arm, not both arms.•Five post-injection plasma samples (2-5 min after ICG bolus) are enough to accurately develop the decay curve for plasma ICG concentration and estimate PV by extrapolation.
血浆容量（PV）可能是健康状况的重要指标，并且可能影响血浆生物标志物的解读，但由于操作复杂且耗时，很少进行测量。吲哚菁绿（ICG）是一种水溶性三碳菁染料，循环半衰期为2 - 3分钟，能够快速清除并可重复使用。它广泛应用于医学诊断测试，包括眼科成像、肝功能和心输出量测量，尤其在重症监护中。ICG已被验证可用于测量人体的PV，然而之前的研究提供的已发表细节极少，或者仅关注该方法的某一个方面。我们旨在制定一份详细的、优化的方案，用于使用ICG测量育龄女性的PV。我们结合了其他研究的最佳实践，并对方案进行了效率优化。•该方法将从采血到确定PV的时间缩短至约2小时，估计PV所需的血浆量减少至2.5毫升（注射ICG前1.5毫升，注射后1.0毫升）。•仅在一侧手臂插入静脉（IV）导管而非双侧手臂，减少了受试者的不便。•注射后五个血浆样本（ICG推注后2 - 5分钟）足以准确绘制血浆ICG浓度的衰减曲线，并通过外推法估计PV。
