Feßler Andrea T, Scholtzek Anissa D, Schug Angela R, Kohn Barbara, Weingart Christiane, Hanke Dennis, Schink Anne-Kathrin, Bethe Astrid, Lübke-Becker Antina, Schwarz Stefan
Institute of Microbiology and Epizootics, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany.
Veterinary Centre for Resistance Research (TZR), Freie Universität Berlin, 14163 Berlin, Germany.
Antibiotics (Basel). 2022 Jan 25;11(2):152. doi: 10.3390/antibiotics11020152.
A total of 215 isolates from infections of dogs and cats, including 49 , 37 , 59 , 56 , and 14 , were investigated for their susceptibility to 27 (Gram-positive bacteria) or 20 (Gram-negative bacteria) antimicrobial agents/combinations of antimicrobial agents by broth microdilution according to the recommendations of the Clinical and Laboratory Standards Institute. Moreover, all isolates were analysed for their susceptibility to the biocides benzalkonium chloride, chlorhexidine, polyhexanide, and octenidine by a recently published broth microdilution biocide susceptibility testing method. While the isolates did not show expanded resistances, considerable numbers of the isolates were resistant to penicillins, macrolides, tetracyclines, and fluoroquinolones. Even a single vancomycin-resistant isolate that carried the gene cluster was detected. Expanded multiresistance phenotypes were also detected among the isolates, including a single carbapenem-resistant, -positive isolate. In addition, multiresistant isolates were detected. The minimal inhibitory concentrations of the biocides showed unimodal distributions but differed with respect to the biocide and the bacterial species investigated. Although there were no indications of a development of biocide resistance, some isolates exhibited benzalkonium MICs higher than the highest test concentration.
共对215株来自犬猫感染的分离株进行了研究，这些分离株包括49株、37株、59株、56株和14株，根据临床和实验室标准协会的建议，通过肉汤微量稀释法检测它们对27种（革兰氏阳性菌）或20种（革兰氏阴性菌）抗菌剂/抗菌剂组合的敏感性。此外，采用最近发表的肉汤微量稀释杀菌剂敏感性试验方法，分析了所有分离株对苯扎氯铵、洗必泰、聚己缩胍和奥替尼啶等杀菌剂的敏感性。虽然这些分离株未表现出广泛耐药性，但相当数量的分离株对青霉素、大环内酯类、四环素类和氟喹诺酮类耐药。甚至检测到一株携带基因簇的耐万古霉素分离株。在这些分离株中还检测到广泛的多重耐药表型，包括一株耐碳青霉烯类、革兰氏阳性分离株。此外，还检测到多重耐药的分离株。杀菌剂的最低抑菌浓度呈单峰分布，但因杀菌剂和所研究的细菌种类而异。虽然没有迹象表明存在杀菌剂耐药性的发展，但一些分离株的苯扎氯铵最低抑菌浓度高于最高测试浓度。
