Müller-Heupt Lena Katharina, Vierengel Nina, Groß Jonathan, Opatz Till, Deschner James, von Loewenich Friederike D
Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, D-55131 Mainz, Germany.
Department of Chemistry, Johannes Gutenberg-University, Duesbergweg 10-14, D-55128 Mainz, Germany.
Antibiotics (Basel). 2022 Jan 31;11(2):186. doi: 10.3390/antibiotics11020186.
Novel plant-derived antimicrobials are of interest in dentistry, especially in the treatment of periodontitis, since the use of established substances is associated with side effects and concerns of antimicrobial resistance have been raised. Thus, the present study was performed to quantify the antimicrobial efficacy of crude plant extracts against , a pathogen associated with periodontitis. The minimal inhibitory concentrations (MICs) of leaf, leaf, root and root extracts were determined by broth microdilution for ATCC 33277 according to CLSI (Clinical and Laboratory Standards Institute). The MICs for the , and extracts ranged from 64 mg/L to 1024 mg/L. The lowest MIC was determined for an ethanolic extract with 4 mg/L. The MIC for the anthraquinone rhein was also measured, as the antimicrobial activity of root extracts can be partially traced back to rhein. Rhein showed a remarkably low MIC of 0.125 mg/L. However, the major compounds of the root extract were not further separated and purified. In conclusion, root extracts should be further studied for the treatment of periodontitis.
新型植物源抗菌剂在牙科领域备受关注，尤其是在牙周炎的治疗方面，因为现有药物的使用存在副作用，且人们对抗菌药物耐药性的担忧也日益增加。因此，本研究旨在量化植物粗提物对与牙周炎相关的病原体的抗菌效果。根据临床和实验室标准协会（CLSI）的方法，采用肉汤微量稀释法测定了[植物名称1]叶、[植物名称2]叶、[植物名称3]根和[植物名称4]根提取物对牙龈卟啉单胞菌ATCC 33277的最低抑菌浓度（MIC）。[植物名称1]、[植物名称2]和[植物名称3]提取物的MIC范围为64 mg/L至1024 mg/L。乙醇提取物的最低MIC为4 mg/L。由于[植物名称4]根提取物的抗菌活性部分可追溯到大黄酸，因此也测定了大黄酸的MIC。大黄酸的MIC极低，为0.125 mg/L。然而，[植物名称4]根提取物的主要化合物并未进一步分离和纯化。总之，[植物名称4]根提取物在牙周炎治疗方面值得进一步研究。
