Jiang Lihua, Wang Qiong, Hu Ning, Yang Jun
School of General Education, Chongqing City Management College, Chongqing 400055, P. R. China.
Department of Basic Medicine, Chongqing Medical and Pharmaceutical College, Chongqing 401331, P. R. China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2022 Feb 25;39(1):112-119. doi: 10.7507/1001-5515.202105060.
Liposomes with precisely controlled composition are usually used as membrane model systems to investigate the fundamental interactions of membrane components under well-defined conditions. Hydration method is the most common method for liposome formation which is found to be influenced by composition of the medium. In this paper, the effects of small alcohol (ethanol) on the hydration of lipid molecules and the formation of liposomes were investigated, as well as its coexistence with sodium chloride. It was found that ethanol showed the opposite effect to that of sodium chloride on the hydration of lipid molecules and the formation of liposomes. The presence of ethanol promoted the formation of liposomes within a certain range of ethanol content, but that of sodium chloride suppressed the liposome formation. By investigating the fluorescence intensity and continuity of the swelled membranes as a function of contents of ethanol and sodium chloride, it was found that sodium chloride and ethanol showed the additive effect on the hydration of lipid molecules when they coexisted in the medium. The results may provide some reference for the efficient preparation of liposomes.
具有精确可控组成的脂质体通常用作膜模型系统，以研究在明确条件下膜成分的基本相互作用。水合方法是脂质体形成最常用的方法，发现其受介质组成的影响。本文研究了小醇（乙醇）对脂质分子水合作用和脂质体形成的影响，以及它与氯化钠共存的情况。发现乙醇对脂质分子水合作用和脂质体形成的影响与氯化钠相反。在一定乙醇含量范围内，乙醇的存在促进了脂质体的形成，而氯化钠的存在则抑制了脂质体的形成。通过研究溶胀膜的荧光强度和连续性随乙醇和氯化钠含量的变化，发现当它们在介质中共存时，氯化钠和乙醇对脂质分子的水合作用表现出加和效应。这些结果可为脂质体的高效制备提供一些参考。
