使用一步法 RT-PCR 和 Sanger 测序快速检测包括奥密克戎在内的 SARS-CoV-2 变体。
Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing.
机构信息
KU Leuven, Rega Institute, Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, 3000 Leuven, Belgium.
KU Leuven, Rega Institute, Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, 3000 Leuven, Belgium; University Hospitals Leuven, Department of Laboratory Medicine, National Reference Center for Respiratory Pathogens, 3000 Leuven, Belgium.
出版信息
J Virol Methods. 2022 Jun;304:114512. doi: 10.1016/j.jviromet.2022.114512. Epub 2022 Mar 4.
SARS-CoV-2 has kept the world in suspense for almost 2 years now. The virus spread rapidly worldwide and several variants of concern have emerged: Alpha, Beta, Gamma, Delta and recently Omicron. A rapid method to detect key mutations is needed because these variants may jeopardize the effectiveness of immune protection following vaccination or past infection. This article describes an easy, cheap and fast method for the detection of mutations in the spike protein that are indicative for specific variants. This method can easily distinguish Omicron from other variants.
SARS-CoV-2 已经让全世界悬而未决近 2 年了。该病毒在全球迅速传播，出现了几种令人关注的变体：阿尔法、贝塔、伽马、德尔塔，最近还有奥密克戎。因此，我们需要一种快速的方法来检测关键突变，因为这些变体可能会影响疫苗接种或既往感染后的免疫保护效果。本文描述了一种用于检测刺突蛋白中特定变体标志性突变的简单、廉价和快速的方法。该方法可以轻松区分奥密克戎和其他变体。
Zotero 插件