Li Keke, Li Zhongyu, Men Lei, Li Jiwen, Gong Xiaojie
College of Life Sciences, Key Laboratory of Biotechnology and Bioresources Utilization, Dalian Minzu University, Ministry of Education, Dalian 116600, China.
School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China.
Foods. 2023 Jan 5;12(2):248. doi: 10.3390/foods12020248.
This study aimed to develop an integrated approach of deep eutectic solvent-based ultrasound-assisted extraction (DES-UAE) to simultaneously extract five major bioactive macamides from the roots of Walp. Ten different DESs containing choline chloride and selected hydrogen-bond donors were prepared and evaluated based on the extracted macamide content determination using high-performance liquid chromatography (HPLC). Choline chloride/1,6-hexanediol in a 1:2 molar ratio with 20% water exhibited the most promising extraction efficiencies under the optimized parameters verified using single-factor optimization as well as Box-Behnken design. Using the optimized DES-UAE method, the extraction efficiencies of the five macamides were up to 40.3% higher compared to those using the most favorable organic solvent petroleum ether and were also superior to those of the other extraction methods, such as heating and combination of heating and stirring. Furthermore, using the macroporous resin HPD-100, the recoveries of the five target macamides from the DES extraction reached 85.62-92.25%. The 20 μg/mL group of the five macamide extracts showed superior neuroprotective activity against PC12 cell injury than that of the positive drug nimodipine. The macamide extracts also showed higher NO inhibition in LPS-stimulated RAW264.7 cells. Thus, the developed approach was a green and potential alternative that can be used to extract bioactive macamide constituents from in the pharmaceutical and food industries.
本研究旨在开发一种基于深共晶溶剂的超声辅助提取（DES-UAE）综合方法，以同时从Walp.的根中提取五种主要生物活性马卡酰胺。制备了十种含有氯化胆碱和选定氢键供体的不同DES，并基于使用高效液相色谱（HPLC）测定提取的马卡酰胺含量进行评估。在使用单因素优化以及Box-Behnken设计验证的优化参数下，摩尔比为1:2且含20%水的氯化胆碱/1,6-己二醇表现出最有前景的提取效率。使用优化的DES-UAE方法，与使用最有利的有机溶剂石油醚相比，五种马卡酰胺的提取效率提高了40.3%，并且也优于其他提取方法，如加热以及加热与搅拌相结合的方法。此外，使用大孔树脂HPD-100，从DES提取物中回收的五种目标马卡酰胺的回收率达到85.62-92.25%。五种马卡酰胺提取物的20μg/mL组对PC12细胞损伤的神经保护活性优于阳性药物尼莫地平。马卡酰胺提取物在LPS刺激的RAW264.7细胞中也表现出更高的NO抑制作用。因此，所开发的方法是一种绿色且有潜力的替代方法，可用于在制药和食品工业中从Walp.中提取生物活性马卡酰胺成分。
