Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, China.
Guangxi University, Nanning, China.
PeerJ. 2022 Mar 8;10:e13042. doi: 10.7717/peerj.13042. eCollection 2022.
is an important economic value snail species with high medicinal value. The gut microbes of aquatic animals plays a vital role in food digestion and nutrient absorption. Herein, we aimed at high-throughput sequencing of 16S rRNA to further investigate whether there were differences in the composition and function of gut microbes of adult and juvenile snails, as well as sediments. This study found that the microbial diversity of the sediment was significantly higher than that of the snails gut ( < 0.001), but there was no significant difference between the gut flora of adult and juvenile snails ( > 0.05). A total of 47 phyla and 644 genera were identified from all samples. Proteobacteria and Verrucomicrobia were the two dominant phyla in all samples, and overall relative abundances was 48.2% and 14.2%, respectively. Moreover, the relative abundances of and in the gut of juvenile snails (30.8%, 11.8%) were higher than those of adults (27.7%, 10.6%) at the genus level ( > 0.05). Then, four indicator genera were found, namely , , and , and they abundance in the gut of juvenile snails was significantly higher than that of adults ( < 0.05). This results imply the potential development of as a bait for juvenile snail openings. We observed that was the primary biomarker of the snail gut and sediments ( < 0.001), and it may be a cellulose-degrading bacteria. Function prediction revealed significantly better biochemical function in the snail gut than sediments ( < 0.001), but no significant differences in adult and juvenile snail ( > 0.05). In conclusion, studies show that the snail gut and sediment microbial composition differ, but the two were very similar. The microbial composition of the snail gut was relatively stable and has similar biological functions. These findings provide valuable information for in-depth understanding of the relationship between snails and environmental microorganisms.
田螺是一种具有重要经济价值的食用蜗牛，同时也具有很高的药用价值。水生动物的肠道微生物在食物消化和营养吸收方面起着至关重要的作用。本研究旨在通过高通量 16S rRNA 测序，进一步探讨成年和幼年田螺以及沉积物中肠道微生物的组成和功能是否存在差异。本研究发现，沉积物的微生物多样性明显高于田螺肠道（<0.001），但成年和幼年田螺肠道菌群之间没有显著差异（>0.05）。从所有样本中总共鉴定出 47 个门和 644 个属。所有样本中两个优势门为变形菌门（Proteobacteria）和疣微菌门（Verrucomicrobia），相对丰度分别为 48.2%和 14.2%。此外，在属水平上，幼年田螺肠道中 （30.8%，11.8%）的相对丰度高于成年田螺（27.7%，10.6%）（>0.05）。然后，发现了四个指示菌属，即 、 、 和 ，它们在幼年田螺肠道中的丰度明显高于成年田螺（<0.05）。这一结果表明，可能可以将 开发为幼年田螺的诱食剂。我们观察到，在田螺肠道和沉积物中， 是主要的生物标志物（<0.001），它可能是一种纤维素降解菌。功能预测显示，田螺肠道的生化功能明显优于沉积物（<0.001），但成年和幼年田螺之间没有显著差异（>0.05）。总之，研究表明田螺肠道和沉积物的微生物组成存在差异，但两者非常相似。田螺肠道的微生物组成相对稳定，具有相似的生物学功能。这些发现为深入了解田螺与环境微生物之间的关系提供了有价值的信息。
