Dai Wanqin, Zhang Junzhe, Wang Yun, Jiao Chunlei, Song Zhuda, Ma Yuhui, Ding Yayun, Zhang Zhiyong, He Xiao
CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Chinese Academy of Sciences, Beijing, China.
CAS-HKU Joint Laboratory of Metallomics on Health and Environment, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China.
Front Toxicol. 2021 Dec 13;3:753316. doi: 10.3389/ftox.2021.753316. eCollection 2021.
Quantifying the distribution of nanomaterials in complex samples is of great significance to the toxicological research of nanomaterials as well as their clinical applications. Radiotracer technology is a powerful tool for biological and environmental tracing of nanomaterials because it has the advantages of high sensitivity and high reliability, and can be matched with some spatially resolved technologies for non-invasive, real-time detection. However, the radiolabeling operation of nanomaterials is relatively complicated, and fundamental studies on how to optimize the experimental procedures for the best radiolabeling of nanomaterials are still needed. This minireview looks back into the methods of radiolabeling of nanomaterials in previous work, and highlights the superiority of the "last-step" labeling strategy. At the same time, the problems existing in the stability test of radiolabeling and the suggestions for further improvement are also addressed.
量化纳米材料在复杂样品中的分布对于纳米材料的毒理学研究及其临床应用具有重要意义。放射性示踪技术是纳米材料生物和环境追踪的有力工具，因为它具有高灵敏度和高可靠性的优点，并且可以与一些空间分辨技术相匹配以进行非侵入性实时检测。然而，纳米材料的放射性标记操作相对复杂，仍需要对如何优化实验程序以实现纳米材料的最佳放射性标记进行基础研究。本综述回顾了以往工作中纳米材料放射性标记的方法，并突出了“最后一步”标记策略的优越性。同时，还讨论了放射性标记稳定性测试中存在的问题以及进一步改进的建议。
