Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
J Am Chem Soc. 2022 Apr 6;144(13):5812-5819. doi: 10.1021/jacs.1c11543. Epub 2022 Mar 18.
Detecting metal ions with a high spatiotemporal resolution is critical to understanding the roles of the metal ions in both healthy and disease states. Although spatiotemporal controls of metal-ion sensors using light have been demonstrated, the lack of penetration depth in tissue and has limited their application. To overcome this limitation, we herein report the use of high-intensity focused ultrasound (HIFU) to remotely deliver on-demand, spatiotemporally resolved thermal energy to activate the DNAzyme sensors at the targeted region both and . A Zn-selective DNAzyme probe is inactivated by a protector strand to block the formation of catalytic enzyme structure, which can then be activated by an HIFU-induced increase in the local temperature. With this design, Zn-specific fluorescent resonance energy transfer (FRET) imaging has been demonstrated by the new DNAzyme-HIFU probes in both HeLa cells and mice. The current method can be applied to monitor many other metal ions for imaging and medical diagnosis using metal-specific DNAzymes that have either been obtained or can be selected using selection.
高时空分辨率检测金属离子对于理解金属离子在健康和疾病状态中的作用至关重要。尽管已经证明了利用光来控制金属离子传感器的时空特性，但由于组织穿透深度有限，它们的应用受到限制。为了克服这一限制，我们在此报告使用高强度聚焦超声（HIFU）远程按需传递时空分辨的热能，以在靶向区域激活 DNA 酶传感器。Zn 选择性 DNA 酶探针被保护链失活以阻止催化酶结构的形成，然后可以通过 HIFU 诱导的局部温度升高来激活。通过这种设计，新型 DNA 酶-HIFU 探针在 HeLa 细胞和小鼠中均实现了 Zn 特异性荧光共振能量转移（FRET）成像。该方法可应用于使用金属特异性 DNA 酶监测许多其他金属离子，这些 DNA 酶可以通过选择或已获得用于成像和医学诊断。
