Hawkins B L, Peterson E M, de la Maza L M
Diagn Microbiol Infect Dis. 1986 Sep;5(3):207-13. doi: 10.1016/0732-8893(86)90003-9.
To evaluate the advantages of agitation in reducing the detection time and increasing the recovery rate of positive blood cultures, 1,000 three-bottle sets of tryptic soy broth on adult inpatients were analyzed. Two bottles were transiently vented, one of which was agitated (250 rpm) for 7-19 hr at 35 degrees C. The other vented bottle and the anaerobic bottle were incubated stationary at 35 degrees C. Smears and subcultures were performed 7-19 hr after collection on both agitated and nonagitated vented bottles. Subcultures were done on all bottles at 72 hr and smears were performed on the anaerobic bottle. There were 137 of 1000 (13.7%) positive cultures from 90 patients. The agitated bottle detected 112 of 137 (81.8%) positive cultures, was the first or only means of detection in 57 of 137 cultures (41.6%), and was the only positive bottle in 30 of 137 (21.9%) cultures. The nonagitated vented bottle detected 89 of 137 (65.0%) of positive cultures and was the only means of detection in 13 of 137 (9.5%), but was never the first means of detection. The anaerobic bottle detected 76 of 137 (55.5%) of positive cultures, was the first or only means of detection in 11 of 137 (8.0%), and was the first means of detection in one of 137 (0.7%) cultures. When both the agitated and nonagitated bottle were positive, the agitated bottle was positive on the average 35 hr earlier. We conclude that agitation of the vented bottle in a conventional blood culture system significantly decreases the detection time of positive blood cultures and increases the number of positive blood cultures detected.
为评估振荡在缩短检测时间及提高阳性血培养回收率方面的优势，对1000套用于成年住院患者的三瓶胰蛋白酶大豆肉汤进行了分析。两瓶进行短暂排气，其中一瓶在35℃下以250转/分钟的速度振荡7至19小时。另一瓶排气瓶和厌氧瓶在35℃下静置培养。在采集样本7至19小时后，对振荡和未振荡的排气瓶进行涂片和传代培养。72小时时对所有瓶子进行传代培养，并对厌氧瓶进行涂片。1000份培养样本中有137份（13.7%）来自90名患者的培养结果呈阳性。振荡瓶检测出137份阳性培养物中的112份（81.8%），在137份培养物中有57份（41.6%）是首个或唯一的检测手段，在137份培养物中有30份（21.9%）是唯一的阳性瓶。未振荡的排气瓶检测出137份阳性培养物中的89份（65.0%），在137份中有13份（9.5%）是唯一的检测手段，但从未是首个检测手段。厌氧瓶检测出137份阳性培养物中的76份（55.5%），在137份中有11份（8.0%）是首个或唯一的检测手段，在137份培养物中有1份（0.7%）是首个检测手段。当振荡瓶和未振荡瓶均为阳性时，振荡瓶平均早35小时呈阳性。我们得出结论，在传统血培养系统中对排气瓶进行振荡可显著缩短阳性血培养的检测时间，并增加检测出的阳性血培养数量。
