Boutahiri Salima, Eto Bruno, Bouhrim Mohamed, Mechchate Hamza, Saleh Asmaa, Al Kamaly Omkulthom, Drioiche Aziz, Remok Firdaous, Samaillie Jennifer, Neut Christel, Gressier Bernard, Kouoh Elombo Ferdinand, Nassiri Laila, Zair Touriya, Sahpaz Sevser
Univ. Lille, University of Liège, University of Picardie Jules Verne, JUNIA, UMRT 1158 BioEcoAgro, Specialized Metabolites of Plant Origin, F-59000 Lille, France.
Research Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, B.P. 11201 Zitoune, Meknes 50070, Morocco.
Life (Basel). 2022 Feb 22;12(3):328. doi: 10.3390/life12030328.
Lavender aqueous extracts are widely used in the Moroccan traditional medicine for their antibacterial properties. However, previous research have generally focused on investigating the antibacterial activity of lavender essential oils. The aim of this study is to evaluate the antibacterial activity of the Moroccan (Mill.) Cav. aqueous extract, alone, as well as in combination with extracts of other plant species known for their antibacterial activity: Spenn., Vahl. and Benth. We have tested the antibacterial activity of , , and aqueous extracts individually and in combination against 34 strains using the agar dilution method. The combination effect was evaluated using the fractional inhibitory concentration (FIC). Polyphenol and tannin contents were determined using Folin-Ciocalteu reagent, and then some phenolic compounds were identified using UHPLC-MS. All the extracts displayed a large spectrum of antibacterial activity, especially against staphylococci, streptococci, and . The minimum inhibitory concentration (MIC) values reached 0.15 ± 0.00 mg/mL for tested with and 0.20 ± 0.07 mg/mL for tested with or . Association of the extract with , and showed synergistic effects (FIC ≤ 1). Moreover, the association of with was active against most of the Gram-negative strains resistant to the individual extracts. Determination of polyphenol and tannin contents showed the richness of the studied plants in these compounds. Additionally, chromatographic analysis demonstrated the high presence of rosmarinic acid in all the studied plant extracts. To our knowledge, this is the first study that shows the enhancing effect of the antibacterial activity of aqueous extract combined with , and . These results confirm the effectiveness of the plant mixtures commonly used by traditional healers in Morocco and suggest that might be used as an antibacterial agent either alone or, more efficiently, in combination with , and .
薰衣草水提取物因其抗菌特性在摩洛哥传统医学中被广泛使用。然而，先前的研究通常集中于调查薰衣草精油的抗菌活性。本研究的目的是评估摩洛哥薰衣草（Mill.）Cav.水提取物单独以及与其他已知具有抗菌活性的植物物种提取物（Spenn.、Vahl.和Benth.）联合使用时的抗菌活性。我们使用琼脂稀释法分别测试了薰衣草、Spenn.、Vahl.和Benth.水提取物单独及联合对34株菌株的抗菌活性。使用分数抑菌浓度（FIC）评估联合效应。使用福林 - 西奥尔特试剂测定多酚和单宁含量，然后使用超高效液相色谱 - 质谱法鉴定一些酚类化合物。所有提取物均表现出广泛的抗菌活性，尤其是对葡萄球菌、链球菌和[此处原文缺失一种菌名]。薰衣草与Spenn.联合测试时的最低抑菌浓度（MIC）值达到0.15±0.00mg/mL，薰衣草与Vahl.或Benth.联合测试时的MIC值达到0.20±0.07mg/mL。薰衣草提取物与Spenn.、Vahl.和Benth.联合显示出协同效应（FIC≤1）。此外，薰衣草与Spenn.联合对大多数对单独提取物耐药的革兰氏阴性菌株有活性。多酚和单宁含量的测定表明所研究的植物富含这些化合物。此外，色谱分析表明所有研究的植物提取物中迷迭香酸含量很高。据我们所知，这是第一项表明薰衣草水提取物与Spenn.、Vahl.和Benth.联合使用可增强抗菌活性的研究。这些结果证实了摩洛哥传统治疗师常用的植物混合物的有效性，并表明薰衣草可单独用作抗菌剂，或者更有效地与Spenn.、Vahl.和Benth.联合使用。
