Hunter E F, Farshy C E, Liska S L, Cruce D D, Crawford J A, Feeley J C
J Clin Microbiol. 1982 Sep;16(3):483-6. doi: 10.1128/jcm.16.3.483-486.1982.
The extraction of Treponema pallidum antigen with sodium desoxycholate, based on a previously described procedure (J. Portnoy and H.J. Magnuson, J. Immuno. 75:348-355, 1955), was used in an enzyme-linked immunosorbent assay (ELISA) test for syphilis. The antigen was prepared from T. pallidum street strain no. 14, and its overall sensitivity and specificity was compared with those of sonicated antigen preparations made with phosphate-buffered saline. The optimum serum dilution for testing and the significant absorbance reading at 490 nm were selected by examination of quantitative dilutions of 91 sera from presumably normal individuals and 92 sera from syphilitics. The time and temperature of serum and conjugate incubations were also examined. With an absorbance reading of greater than or equal to 0.2 at the 1:80 serum dilution, 88 (95.8%) of 92 sera from syphilitics were reactive in the ELISA test with desoxycholate-extracted antigen, and 82 (89.1%) were reactive with the sonicated antigen. Only one nonsyphilitic serum was reactive with each antigen. Greater sensitivity without loss in specificity was obtained with longer serum and conjugate incubations. We concluded that an ELISA test with sodium desoxycholate-extracted antigen is more sensitive than and equally specific to an ELISA with sonicated treponemal antigen.
基于先前描述的方法（J. 波特诺伊和H.J. 马格努森，《免疫学杂志》75:348 - 355，1955年），用脱氧胆酸钠提取梅毒螺旋体抗原，用于梅毒的酶联免疫吸附测定（ELISA）试验。该抗原由梅毒螺旋体14号街毒株制备，并将其总体敏感性和特异性与用磷酸盐缓冲盐水制备的超声破碎抗原制剂的敏感性和特异性进行比较。通过检测91份来自假定正常个体的血清和92份来自梅毒患者的血清的定量稀释液，选择检测的最佳血清稀释度和490nm处的显著吸光度读数。还检测了血清和结合物孵育的时间和温度。在血清稀释度为1:80时，吸光度读数大于或等于0.2，92份梅毒患者血清中的88份（95.8%）在ELISA试验中与脱氧胆酸钠提取的抗原反应，82份（89.1%）与超声破碎抗原反应。每种抗原仅一份非梅毒血清有反应。延长血清和结合物孵育时间可获得更高的敏感性且不损失特异性。我们得出结论，用脱氧胆酸钠提取抗原的ELISA试验比用超声破碎梅毒螺旋体抗原的ELISA试验更敏感且特异性相同。
