Division of Nephrology, Washington University in St. Louis School of Medicine, St. Louis, Missouri.
Department of Developmental Biology, Washington University in St. Louis School of Medicine, St. Louis, Missouri.
Kidney360. 2021 May 14;2(7):1196-1204. doi: 10.34067/KID.0001822021. eCollection 2021 Jul 29.
Single-cell RNA-sequencing (scRNA-seq) has been widely adopted in recent years due to standardized protocols and automation, reliability, and standardized bioinformatic pipelines. The most widely adopted platform is the 10× Genomics solution. Although powerful, this system is limited by its high cost, moderate throughput, and the inability to customize due to fixed kit components. This study will cover new approaches that do not rely on microfluidics and thus have low entry costs, are highly customizable, and are within the reach of any laboratory possessing molecular biology expertise.
单细胞 RNA 测序（scRNA-seq）近年来由于标准化的方案和自动化、可靠性和标准化的生物信息学管道而得到广泛应用。应用最广泛的平台是 10× Genomics 解决方案。尽管功能强大，但该系统受到高成本、中等通量和由于固定试剂盒组件而无法定制的限制。本研究将涵盖不依赖于微流控技术的新方法，因此具有较低的进入成本、高度可定制且任何拥有分子生物学专业知识的实验室都能够实现。
